| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Gergely, Csilla
University of Montpellier
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (9/9 displayed)
- 2019Peptide-route functionalization of chalcogenide films
- 2018Elaboration and Characterization of Porous Silicon multilayer for biomaterial applications
- 2018Elaboration and Characterization of Porous Silicon multilayer for biomaterial applications
- 2014Morphology and nanomechanics of mice sensory neurons following peripheral nerve injury
- 2014Sprayed Cells and Polyelectrolyte Films for Biomaterial Functionalization: The Influence of Physical PLL-PGA Film Treatments on Dental Pulp Cell Behaviorcitations
- 2013Morphology and Nanomechanics of Sensory Neurons Growth Cones following Peripheral Nerve Injurycitations
- 2013Nanomechanics of mice sensory neurons as measured by atomic force microscopy.
- 2011Dental Pulp Stem Cells Adhesion/Proliferation On Porous Silicon Scaffold
- 2008Specific peptide for functionalization of GaN - art. no. 699121
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document
Dental Pulp Stem Cells Adhesion/Proliferation On Porous Silicon Scaffold
Abstract
Porous silicon (pSi) is a promising biomaterial that is non-toxic and bioresorbable. Surface modifications can offer control over the degradation rate and can also impart properties that promote cell adhesion. Coupling the capacities of Dental Pulp Stem Cells (DPSC) with the pSi properties is a promising tool in regenerative medicine. P-type silicon wafers were etched at a constant current density of 30 mA/cm2 or 300 mA/cm2. The samples were oxidized or hydrosililzed. The topography of surface modified pSi samples was analysed by scanning electronic microscope (SEM) and water contact angle measurement. Dental pulp cells were collected from healthy adults and analyzed by flow cytometry. Cells were incubated on pSi samples for either 4 hours or 24 hours. Cellular morphology on pSi was evaluated with fluorescein diacetate (FDA) staining. Cell proliferation was measured through acid phosphatase activity. After oxidation or hydrosililation, at either 30 or 300 mA/cm2, pSi wafers became clearly hydrophilic. SEM revealed a highly porous surface, with a mean size of pore of 10nm±2 for 30mA/cm2, and 21nm±3 for 300mA/cm2. With flow cytometry, cells were 17% CD34+ and 77% CD146+. Acid phosphatase assay showed that samples etched with 300mA/cm2 tend to offer a better adhesion for cells; the same tendency was observed for hydrosililation treatment. Cells presented the same morphology on pSi as on culture plate. Surface modification, by turning pSi from hydrophobic to hydrophilic, allows cell adhesion. The two tested sizes of pore and the two tested surface treatments allowed adhesion of DPSC. Cell morphology on pSi was similar to culture plates. pSi resorption time is influenced by surface modification and DPSC adhesion is possible on both surface modifications, highlighting an interest for cell/tissue graft. And the tunable size of pore might permit to incorporate growth factors or nutriments inside the scaffold.