| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Van Vlierberghe, Sandra
Ghent University
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (27/27 displayed)
- 2024Tailorable acrylate-endcapped urethane-based polymers for precision in digital light processing : versatile solutions for biomedical applicationscitations
- 2024Toward in vitro vascular wall models : digital light processing of acrylate‐edcapped urethane‐based polymers into tubular constructs
- 2023Printability evaluation of UV-curable aqueous laponite/urethane-based PEG inkscitations
- 2023Volumetric printing of thiol‐ene photo‐cross‐linkable poly(ε‐caprolactone) : a tunable material platform serving biomedical applicationscitations
- 2023Solid‐state crosslinkable, shape‐memory polyesters serving tissue engineeringcitations
- 2023Microstructured Optical Fiber Made From Biodegradable and Biocompatible Poly(D,L-Lactic Acid) (PDLLA)citations
- 2023Photo-crosslinkable biodegradable polymer coating to control fertilizer releasecitations
- 2022Environmental and economic sustainability of crack mitigation in reinforced concrete with SuperAbsorbent polymers (SAPs)citations
- 2021Increasing the Microfabrication Performance of Synthetic Hydrogel Precursors through Molecular Designcitations
- 2021Increasing the microfabrication performance of synthetic hydrogel precursors through molecular designcitations
- 2021Atomic layer deposition on polymer thin films : on the role of precursor infiltration and reactivitycitations
- 2020Laser welding of carbon fibre filled polytetra fluoroethylenecitations
- 2020The contribution of elastic wave NDT to the characterization of modern cementitious mediacitations
- 2019Amorphous random copolymers of lacOCA and manOCA for the design of biodegradable polyesters with tuneable propertiescitations
- 2019Amorphous random copolymers of lacOCA and manOCA for the design of biodegradable polyesters with tuneable propertiescitations
- 2019Poly(D, L-Lactic Acid) (PDLLA) Biodegradable and Biocompatible Polymer Optical Fibercitations
- 2018Clear to clear laser welding for joining thermoplastic polymers: A comparative study based on physicochemical characterizationcitations
- 2018Ultrathin Poly-DL-Lactic Membranes for Corneal Endothelial Transplantation
- 2018Poly(methyl methacrylate) capsules as an alternative to the ‘’proof-of-concept’’ glass capsules used in self-healing concrete
- 2018Localized optical- quality doping of graphene on silicon waveguides through a TFSA- containing polymer matrixcitations
- 2018Ring opening copolymerisation of lactide and mandelide for the development of environmentally degradable polyesters with controllable glass transition temperaturescitations
- 2017Combinatory approach of methacrylated alginate and acid monomers for concrete applicationscitations
- 2017Characterization of methacrylated polysaccharides in combination with amine-based monomers for application in mortarcitations
- 2016Indirect solid freeform fabrication of an initiator-free photocrosslinkable hydrogel precursor for the creation of porous scaffoldscitations
- 2016Deep proton writing with 12 MeV protons for rapid prototyping of microstructures in polymethylmethacrylatecitations
- 2016Chapter 21 – Biodegradable polyesters: from monomer to application
- 2011Gelatin functionalization of biomaterial surfaces : strategies for immobilization and visualizationcitations
Places of action
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article
Ultrathin Poly-DL-Lactic Membranes for Corneal Endothelial Transplantation
Abstract
Purpose : Corneal endothelial transplantation is limited by a donor shortage, making transplant lists long. As hypothesized in 1978, tissue-engineering could overcome this hurdle by growing corneal endothelial cells (CEnCs) on transplantable scaffolds. Despite the global interest, no tissue-engineered graft has made it yet to the clinic in part due to challenging properties scaffolds must fulfil. This study investigates the use of Poly-DL-lactic (PDLLA) membranes as scaffolds to aid in production of corneal endothelial grafts.<br/><br/>Methods : Membranes were synthesized by spincoating a 10 w/v% solution of gelatin type A on glass as a sacrificial layer (Fig 1A). PDLLA was spincoated on top and plasma treated for surface activation. Gelatin B derivatives, gelatine-methacrylamide (gel-MOD) and gel-MOD-2-aminoethyl methacrylate (gel-MOD-AEMA) were analyzed using Nuclear Magnetic Resonance (NMR) spectroscopy, spincoated on the PDLLA and UV crosslinked (Fig 1B). The membranes were characterized with x-ray photoemission spectroscopy (XPS), optical profilometry (OP) to determine thickness and glucose diffusion. Also, cytocompatibility was investigated using primary and immortalized CEnCs followed by immunocytochemistry.<br/><br/>Results : NMR spectra confirmed the modification of gel-MOD(-AEMA). The presence of stable gelatin layers during the synthesis was confirmed using XPS. The developed membranes exhibited thicknesses ranging from 1.1 - 2.6 µm as obtained via OP and the PDLLA showed a diffusion glucose coefficient of 0.0161cm/s. Finally, the membranes were seeded with primary and immortalized CEnCs and exhibited the characteristic hexagonal shape. Immunocytochemistry showed expression of Na+/K+ ATPase and ZO-1 along the cell membranes of primary and immortalized CEnCs cultured on both culture plastic and gel-MOD(-AEMA) membranes.<br/><br/>Conclusions : In this study, membranes were constructed using PDLLA for mechanical strength and functionalized gelatins to mimic the extracellular matrix. The membranes displayed glucose permeability and because of the ultrathin dimensions they qualify as scaffolds for endothelial keratoplasty. Cultured endothelial cells expressed hallmark proteins representing their pumping and barrier function. Therefore, these novel ultrathin membranes are potentially a suitable scaffold for corneal endothelial tissue engineering required to alleviate the donor shortage.<br/><br/>This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.