| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Van Erps, Jurgen
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (21/21 displayed)
- 2023VCSEL wavelength tunability using controlled mechanical strain
- 2021Increasing the Microfabrication Performance of Synthetic Hydrogel Precursors through Molecular Designcitations
- 20203D direct laser writing of microstructured optical fiber tapers on single-mode fibers for mode-field conversioncitations
- 2018Ultrathin Poly-DL-Lactic Membranes for Corneal Endothelial Transplantation
- 2018Localized optical- quality doping of graphene on silicon waveguides through a TFSA- containing polymer matrixcitations
- 2016Replication of self-centering optical fiber alignment structures using hot embossingcitations
- 2016Hot-embossing replication of self-centering optical fiber alignment structures prototyped by deep proton writingcitations
- 2016Deep proton writing with 12 MeV protons for rapid prototyping of microstructures in polymethylmethacrylatecitations
- 2016Optofluidic multi-measurement system for the online monitoring of lubricant oilcitations
- 2016Design and prototyping of self-centering optical single-mode fiber alignment structurescitations
- 2015Mould insert fabrication of a single-mode fibre connector alignment structure optimized by justified partial metallizationcitations
- 2013Low-coherence interferometry with polynomial interpolation on Compute Unified Device Architectur-enabled graphics processing units
- 2013Gloss, hydrophobicity and surface texture of papers with organic nanoparticle coatings
- 2013B-Calm: An open-source multi-GPU-based 3D-FDTD with multi-pole dispersion for plasmonics
- 2010Populating multi-fiber fiberoptic connectors using an interferometric measurement of fiber tip position and facet quality
- 2010Design and fabrication of embedded micro-mirror inserts for out-of-plane coupling in PCB-level optical interconnects
- 2008Hot embossing of microoptical components prototyped by deep proton writing
- 2008Embedded Micro-Mirror inserts for optical printed circuit boards
- 2008Deep Proton Writing: A tool for rapid prototyping of polymer micro-opto-mechanical modules
- 2007Deep Proton Writing: A tool for rapid prototyping polymer micro-opto-mechanical modules
- 2006Laser Ablation of Parallel Optical Interconnect Waveguides
Places of action
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article
Ultrathin Poly-DL-Lactic Membranes for Corneal Endothelial Transplantation
Abstract
Purpose : Corneal endothelial transplantation is limited by a donor shortage, making transplant lists long. As hypothesized in 1978, tissue-engineering could overcome this hurdle by growing corneal endothelial cells (CEnCs) on transplantable scaffolds. Despite the global interest, no tissue-engineered graft has made it yet to the clinic in part due to challenging properties scaffolds must fulfil. This study investigates the use of Poly-DL-lactic (PDLLA) membranes as scaffolds to aid in production of corneal endothelial grafts.<br/><br/>Methods : Membranes were synthesized by spincoating a 10 w/v% solution of gelatin type A on glass as a sacrificial layer (Fig 1A). PDLLA was spincoated on top and plasma treated for surface activation. Gelatin B derivatives, gelatine-methacrylamide (gel-MOD) and gel-MOD-2-aminoethyl methacrylate (gel-MOD-AEMA) were analyzed using Nuclear Magnetic Resonance (NMR) spectroscopy, spincoated on the PDLLA and UV crosslinked (Fig 1B). The membranes were characterized with x-ray photoemission spectroscopy (XPS), optical profilometry (OP) to determine thickness and glucose diffusion. Also, cytocompatibility was investigated using primary and immortalized CEnCs followed by immunocytochemistry.<br/><br/>Results : NMR spectra confirmed the modification of gel-MOD(-AEMA). The presence of stable gelatin layers during the synthesis was confirmed using XPS. The developed membranes exhibited thicknesses ranging from 1.1 - 2.6 µm as obtained via OP and the PDLLA showed a diffusion glucose coefficient of 0.0161cm/s. Finally, the membranes were seeded with primary and immortalized CEnCs and exhibited the characteristic hexagonal shape. Immunocytochemistry showed expression of Na+/K+ ATPase and ZO-1 along the cell membranes of primary and immortalized CEnCs cultured on both culture plastic and gel-MOD(-AEMA) membranes.<br/><br/>Conclusions : In this study, membranes were constructed using PDLLA for mechanical strength and functionalized gelatins to mimic the extracellular matrix. The membranes displayed glucose permeability and because of the ultrathin dimensions they qualify as scaffolds for endothelial keratoplasty. Cultured endothelial cells expressed hallmark proteins representing their pumping and barrier function. Therefore, these novel ultrathin membranes are potentially a suitable scaffold for corneal endothelial tissue engineering required to alleviate the donor shortage.<br/><br/>This is an abstract that was submitted for the 2018 ARVO Annual Meeting, held in Honolulu, Hawaii, April 29 - May 3, 2018.