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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Ali, M. A. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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Vinken, Mathieu
Vrije Universiteit Brussel
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (5/5 displayed)
- 2024The application of natural language processing for the extraction of mechanistic information in toxicologycitations
- 2023Protocol for designing INVITES-IN, a tool for assessing the internal validity of in vitro studiescitations
- 2016Immunohisto- and cytochemistry analysis of connexins
- 2012Effects of RNA interference-mediated suppression of connexin43 production on the expression of differentiation markers in cultures of adult primary rat hepatocytes
- 2010The effect of the histone deacetylase inhibitor Trichostatin A on the metabolome of cultured primary hepatocytes
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booksection
Immunohisto- and cytochemistry analysis of connexins
Abstract
Immunohistochemistry (IHC) is a ubiquitous used technique to identify and analyze protein expression in the context of tissue and cell morphology. In the connexin research field, IHC is applied to identify the subcellular location of connexin proteins, as this can be directly linked to their functionality. The present chapter describes a protocol for fluorescent IHC to detect connexin proteins in tissues slices and cells, with slight modifications depending on the nature of biological sample, histological processing and/or protein expression level. Basically, fluorescent IHC is a short, simple and cost-effective technique, which allows the visualization of proteins based on fluorescent-labeled antibody-antigen recognition.