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Pasupathy, Karthikeyan
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document
Single-molecule study of dendrimer-hydrocarbon interaction
Abstract
<p>In this paper we report our study on the interaction of dendrimer and hydrocarbon. The absorbance of generation-3 dendrimer PAMAM (0.25%) was reduced by ∼5% in 60 min with the addition of hydrocarbon Squalane (0.405 mg/mL), possibly due to the phase separation between the PAMAM remained in the solution and the PAMAM bound to the Squalane. Using single-molecule fluorescence microscopy we found that PAMAM molecules in the solution could be immobilized by Squalane molecules coated on a glass substrate, as indicated by the much increased fluorescence intensity of the TRITC-labeled PAMAM in an evanescent wave field. Our study offers the first evidence at the single-molecule level on dendrimer and hydrocarbon interaction and facilitates our understanding of using dendrimers for the prevention of gas hydrates for the petroleum industry.</p>