| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Lotery, Andrew
University of Southampton
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (6/6 displayed)
- 2019A lasered mouse model of retinal degeneration displays progressive outer retinal pathology providing insights into early geographic atrophycitations
- 2014Development of a novel bio-compatible polymer film for use as a Bruch’s membrane substitute
- 2011Optimisation of polymer scaffolds for retinal pigment epithelium (RPE) cell transplantation.citations
- 2009Optimisation of polymer scaffolds for ocular cell transplantation
- 2007Fine-scale linkage disequilibrium mapping of age-related macular degeneration in the complement factor H gene regioncitations
- 2001Variation of codons 1961 and 2177 of the Stargardt disease gene is not associated with age-related macular degeneration
Places of action
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document
Development of a novel bio-compatible polymer film for use as a Bruch’s membrane substitute
Abstract
Purpose: To develop and optimise a co-polymer blend of poly(ethylene glycol methacrylate) (PEGM) and poly(methyl methacrylate) (PMMA) to act as a synthetic Bruch’s membrane (BrM) scaffold for primary murine retinal pigment epithelium (RPE) monolayers, as a potential treatment for dry age-related macular degeneration (AMD). This bio-compatible polymer film is designed to mimic BrM and to allow RPE cells to attach to the surface and proliferate, whilst retaining the necessary properties of human BrM, including porosity, thickness, biocompatibility and adhesion for the anchorage-dependant RPE cells. Methods: PEGM and PMMA are biocompatible polymers which are already in use for clinical applications. This co-polymer blend is functionalised at the PEGM site with N,N’-Disuccinimidyl carbonate and a RGDS peptide to facilitate RPE attachment. Electrospinning is used to produce polymer fibre sheets. By altering the parameters of the electrospinning apparatus (voltage, distance from needle to collector, flow rate) we can control the polymer fibre production. Results: By using the electrospinning technique, the co-polymer mat provides the necessary porosity, and by altering the parameters of the electrospinning, the thickness has been optimised to match human BrM (confirmed via an optical profiler, which found that the co-polymer had a thickness of approximately 12µm). The fidelity and effectiveness of the PMMA:PEGM co-polymer have been tested using long-term cultures of primary murine RPE monolayers. Our experiments also characterised RPE-barrier properties to establish the suitability of this novel synthetic scaffold for future transplantation studies Conclusions: This work demonstrates synthesis of a PMMA:PEGM co-polymer film, through the use of electrospinning, which mimics the properties of the BrM (porosity, thickness, biocompatibility and adhesion). This suggests that the electrospun membrane is a viable scaffold to facilitate delivery of RPE cells to the sub-retinal space. This is an exciting prospect and future ...