• Hansen, Tina Beck
• Birk, Tina
• Henriksen, Sidsel
• Buschhardt, Tasja
• Aabo, Søren

Abstract

Food composition, buffer capacity, and fat and protein content have been shown to effect the gastric acid survival of pathogens (Waterman & Small 1998). In this study, simple food-model substances with different buffer capacities were investigated for their ability to support survival of stationary phase Salmonella Typhimurium during simulated gastric acid passage. We used a computer-controlled fermentor to employ pH changes in synthetic gastric fluid, mimicking the dynamic pH during gastric passage. In order to minimise variation, Salmonella enterica serovar Typhimurium was contained in dialysis tubes, enabling simultaneous testing of biological triplicates under varying conditions. Surprisingly, we found that less buffered media provided higher protection of Salmonella, compared to media with high buffer capacity. By investigating the relative gene expression of rpoS and ompR encoding for two major stationary phase ATR regulators, we found an approx. four-fold increase in expression of ompR and an approx. three-fold increase of rpoS in saline and buffered saline, respectively, after 15 min of gastric acid challenge. The relative expression of these genes, were significantly lower in Brain Heart Infusion Broth having a higher buffer capacity. We suggest this to be associated with a varying ability of Salmonella Typhimurium to mount a stationary phase acid tolerance response (ATR) depending on the buffer capacity of the food vehicle.

Topics

• phase
• laser emission spectroscopy
• dialysis