| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Mayer, Philipp
Technical University of Denmark
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (15/15 displayed)
- 2024Determining ecotoxicity drivers and biodegradation kinetics of discharged chemicals in produced water from oil and gas extraction in the North Sea
- 2018Assessing PCB pollution in the Baltic Sea - An equilibrium partitioning based studycitations
- 2018Headspace passive dosing for dose-response testing of volatile hydrophobic organic chemicals
- 2015Comparison of passive and standard dosing of polycyclic aromatic hydrocarbons to the marine algae Phaeodactylum tricornutum
- 2015Equilibrium passive sampling as a tool to study polycyclic aromatic hydrocarbons in Baltic Sea sediment pore-water systemscitations
- 2014The effect of humic acids on biodegradation of polycyclic aromatic hydrocarbons depends on the exposure regimecitations
- 2013Baseline Toxic Mixtures of Non-Toxic Chemicalscitations
- 2013The dosing determines mutagenicity of hydrophobic compounds in the Ames II assay with metabolic transformationcitations
- 2012Recreating the seawater mixture composition of HOCs in toxicity tests with Artemia franciscana by passive dosingcitations
- 2011A Contaminant Trap as a Tool for Isolating and Measuring the Desorption Resistant Fraction of Soil Pollutantscitations
- 2011Application of passive dosing to study the biotransformation and biodegradation of hydrophobic compounds
- 2011Application of passive dosing to study the biotransformation and biodegradation of hydrophobic
- 2010Controlling and maintaining exposure of hydrophobic organic compounds in aquatic toxicity tests by passive dosingcitations
- 2010Passive Dosing for Producing Defined and Constant Exposure of Hydrophobic Organic Compounds during in Vitro Toxicity Testscitations
- 2009In Situ Silicone Tube Microextractioncitations
Places of action
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document
Application of passive dosing to study the biotransformation and biodegradation of hydrophobic compounds
Abstract
Achieving well-defined and constant dissolved concentrations of hydrophobic compounds is challenging due to volatilization or sorptive losses. With passive dosing, continual partitioning into the test medium of compound(s) loaded in a polymer compensates for losses, and provides defined and constant dissolved concentrations. Passive dosing can be used for studying biotransformation/ degradation. Here, the polymer HOC reservoir also compensates for losses due to the bio-transformation/degradation process itself. Furthermore, a large mass of test compound is introduced so that compound turnover is significant even at low dissolved concentrations thus facilitating measurement of the relevant endpoint (e.g., metabolic products in biotransformation or growth in biodegradation). This study details two applications of passive dosing for studying bio-transformation/degradation. A format has been developed to study the biodegradation of phenanthrene and fluoranthene by the bacterial strain EPA 505, allowing degradation rates to be quantified at defined freely dissolved concentrations from mg/L down to ng/L levels. Passive dosing was also applied for quantifying the mutagenicity of benzo(a)pyrene metabolites produced after activation by the liver S9 mix in the in vitro Ames II assay. Compared to the case with spiking, responses from passive dosing were shifted by a factor 100-1000 to lower concentrations, and were also more reproducible between repeated tests. This difference in apparent sensitivity cannot solely be explained by partitioning, and is due to slow dissolution kinetics as well as massdepletion of the spiked benzo(a)pyrene. Therefore, passive dosing is a useful tool for the study of hydrophobic compound bio-transformation/degradation at well-defined dissolved concentrations down to very low levels. Important advantages include studying process kinetics at precisely defined dissolved concentrations and allowing increased compound turnover even at constant and low concentrations.