| People | Locations | Statistics |
|---|---|---|
| Naji, M. |
| |
| Motta, Antonella |
| |
| Aletan, Dirar |
| |
| Mohamed, Tarek |
| |
| Ertürk, Emre |
| |
| Taccardi, Nicola |
| |
| Kononenko, Denys |
| |
| Petrov, R. H. | Madrid |
|
| Alshaaer, Mazen | Brussels |
|
| Bih, L. |
| |
| Casati, R. |
| |
| Muller, Hermance |
| |
| Kočí, Jan | Prague |
|
| Šuljagić, Marija |
| |
| Kalteremidou, Kalliopi-Artemi | Brussels |
|
| Azam, Siraj |
| |
| Ospanova, Alyiya |
| |
| Blanpain, Bart |
| |
| Ali, M. A. |
| |
| Popa, V. |
| |
| Rančić, M. |
| |
| Ollier, Nadège |
| |
| Azevedo, Nuno Monteiro |
| |
| Landes, Michael |
| |
| Rignanese, Gian-Marco |
|
Dufva, Martin
Technical University of Denmark
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (8/8 displayed)
- 2022Customized 3D-printed stackable cell culture inserts tailored with bioactive membranescitations
- 2021Photolithographic Patterning of FluorAcryl for Biphilic Microwell-Based Digital Bioassays and Selection of Bacteriacitations
- 2020Homogeneous circle-to-circle amplification for real-time optomagnetic detection of SARS-CoV-2 RdRp coding sequencecitations
- 2016Enhanced Differentiation of Human Embryonic Stem Cells Toward Definitive Endoderm on Ultrahigh Aspect Ratio Nanopillarscitations
- 2014Reagent deposition for rapid multiplex pathogen identification in human blood culture samples
- 2013Single nucleotide polymorphism (SNP) detection on a magnetoresistive sensor
- 2013Ligation-based mutation detection and RCA in surface un-modified OSTE+ polymer microfluidic chamberscitations
- 2010Microfluidic DNA microarrays in PMMA chips: streamlined fabrication via simultaneous DNA immobilization and bonding activation by brief UV exposurecitations
Places of action
| Organizations | Location | People |
|---|
conferencepaper
Reagent deposition for rapid multiplex pathogen identification in human blood culture samples
Abstract
Blood stream infections led to 135,000 deads annually in EU and fast treatment significantly increases the survival rate. This condition is diagnosed by means of blood cultures (19 Mill blood cultures are drawn annually in EU). In this work, a multiplex peptide nucleic acid / fluorescence in-situ hybridization assay is used (PNA-FISH) for diagnosis of hospital acquired bacteria, such as staphylococcus aureus, enterococcus faecalis, E. coli, candida albicans etc. The test covers 90-95 % of the species by prevalence. It is based on a microscope<br/>slide format with inspection of the fluorescing bacteria viewed in a dual-color microscope configuration. The test takes 20-30 min to perform.<br/>In order to lower the cost of the test, rapid automated reagent deposition is needed. Here, ultrasonic spray coating of polyvinyl alcohol/PNA-probes on microscope glass slides is presented. Different wetting regimes are explored in order to control the spot profile from convex to concave. The spray coated test are compared with manually prepared tests to ensure that the same performance is obtained.