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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Compère, C.
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (10/10 displayed)
- 2017Biofilms and corrosion of stainless alloys in sea water Multidisciplinary characterization of the biofilm
- 2017Infrared sensor for water pollution and monitoring
- 2012Surface enhanced infrared absorption (SEIRA) spectroscopy using gold nanoparticles on As2S3 glasscitations
- 2010Proteomic approaches applied to adhesion factors in marine biofilm-forming bacteria
- 2008Antifouling Properties of Poly(methyl methacrylate) Films Grafted with Poly(ethylene glycol) Monoacrylate Immersed in Seawatercitations
- 2003Sedimentation and fouling of optical surfaces at the ANTARES sitecitations
- 2002Electrochemical impedance spectroscopy of a free-standing oxide filmcitations
- 2002Time-of-flight secondary ion mass spectrometry: characterisation of stainless steel surfaces immersed in natural seawatercitations
- 2001Influence of stainless steel surface treatment on the oxygen reduction reaction in seawatercitations
- 2000Adsorption of proteins on an AISI 316 stainless-steel surface in natural seawater
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document
Proteomic approaches applied to adhesion factors in marine biofilm-forming bacteria
Abstract
Biofouling is ubiquitous in marine environment, and bacteria are among the first organisms to foul surfaces. They form biofilms which serve as focus for the attachment and growth of other organisms, such as invertebrates, sessile plants, and animals (Davis et al., 1989). Mature marine biofouling communities are complex, highly dynamic ecosystems (Fig.1) and once established are extremely difficult to eradicate (Holmstrom et al., 2002). For this reason the understanding of the mechanisms leading to marine bacterial attachment and its subsequent biofilm development are of great biological importance with obvious potential industrial outcomes. This development is conditioned by complex processes involving bacterial attachment to surfaces, growth, cell-to-cell communication, mobility and production of exoproducts constituting the biofilm matrix. Concerning attachment, the molecular strategies used by bacteria are diverse. They can employ pili, fimbria and a plethora of proteins regrouped under the term “adhesins” that recognize many different elements of the target (living or inert) surfaces (Pizarro-Cerda and Cossart, 2006; Fronzes et al., 2008). Although there is consistent data on human pathogenic bacteria attachment mechanisms, scarce information is available for marine bacteria. For this reason this project focused in the marine biofilm-forming bacterium Pseudoalteromonas sp. D41 (P. D41). This organism displays strong and competitive adhesion onto a wide variety of substrates, promoting subsequent biofilm development. Previous physicochemical studies in this strain related the high outer-shell protein content to its adhesion properties (Pradier et al., 2005; Leroy et al., 2008). For this reason, we attempted to unravel the molecular mechanisms responsible for these adhesive and competitive properties though a proteomic strategy, with particular attention to the outer membrane (OM) fraction.