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Medlicott, Natalie
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article
pH dependent polymer surfactants for hindering BSA adsorption to oil-water interface
Abstract
Bovine serum albumin (BSA) adsorption to oil-water interfaces was studied in the presence and absence of two linear polymers poly(dimethylamino)ethyl methacrylate (PDMAEMA) and PDMAEMA-block-[poly butyl acetate]<sub>38700</sub>-PDMAEMA<sub>33000</sub> (PBuA). As the hydrophobicity of the polymers is pH dependent, the pH was varied using pH 6.6, 7.0 and 7.4 during the measurements. Pendant drop and a rheometer with double wall ring geometry were utilized for surface tension and oscillatory shear measurements, respectively. The presence of both the PDMAEME and the PBuA resulted in decreased adsorption of BSA with increasing pH where PBuA was more efficient. The greatest reduction of the interfacial complex viscosity was reached in the presence of PBuA at pH 7.4.