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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Petrov, R. H. | Madrid |
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Kočí, Jan | Prague |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Kim, Yongtae
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document
Multiplex SNP analysis microdevice using allele-specific polymerase chain reaction-microarray
Abstract
In this study, we developed an allele-specific polymerase chain reaction-microarray (AS PCR-microarray) chip to perform the allele-specific gene amplification and microarray-based detection on a single wafer to identify Korean indigenous beef cattle, called Hanwoo. For allele-specific discrimination and amplification, Hanwoo-specific ten homozygous single nucleotide polymorphism (SNP) sites were chosen and analyzed by AS PCR on a chip, and the resultant amplicons were verified by the downstream microarray assay. We could identify Hanwoo with 99.3% reliability by detecting the fluorescence signals of the microarray less than three among eleven spots.