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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Adam, Vojtech
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (4/4 displayed)
- 2023Silver Nanoparticle‐Decorated Reduced Graphene Oxide Nanomaterials Exert Membrane Stress and Induce Immune Response to Inhibit the Early Phase of HIV‐1 Infectioncitations
- 2023Synthesis and characterization of TiO2 nanoparticles combined with geraniol and their synergistic antibacterial activitycitations
- 2019Application of the Enzymatic Electrochemical Biosensors for Monitoring Non-Competitive Inhibition of Enzyme Activity by Heavy Metals ; Aplikace enzymatických elektrochemických biosenzorů pro monitorování nekompetitivní inhibice enzymové aktivity těžkými kovycitations
- 2019A simple electrochemical biosensor for the detection of methylated DNA and for methyltransferase activity monitoring
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document
A simple electrochemical biosensor for the detection of methylated DNA and for methyltransferase activity monitoring
Abstract
DNA methylation is one of the well-known epigenetic mechanism which plays a crucial role in the development of various diseases, such as cancer, cardiovascular diseases or diabetes. Electrochemical biosensors promise excellent results for clinical diagnostics, especially in term of their sensitivity, stability, selectivity, portability, they are cost-effective, easy-to-use and provides a fast response. In this study, simply, reliably and selective DNA based electrochemical biosensor for detection of methylated DNA was fabricated. The proposed biosensor was modified with the synthesised reduced graphene oxide combined with gold nanoparticles (rGO-AuNPs). This nanocomposite has shown a strong affinity to the DNA probe and demonstrated promising analytical characteristics. The electrochemical impedance spectroscopy (EIS) was used for the characterization of interface properties of the gold electrode (GE). Additionally, the sensitivity of the developed biosensor was performed by differential pulse voltammetry (DPV) to investigate the activity of enzyme methyltransferase M.SssI (MTase). Fabricated biosensor offers quite a low detection limit (LOD), which was 3.2 U/ml and limit of quantification (LOQ) was 3.3 U/ml.