| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Svendsen, Winnie Edith
Technical University of Denmark
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (14/14 displayed)
- 2021Continuous Microfluidic Production of Citrem-Phosphatidylcholine Nano-Self-Assemblies for Thymoquinone Deliverycitations
- 2018Paper-based sensors for rapid detection of virulence factor produced by Pseudomonas aeruginosacitations
- 2018Paper-based sensors for rapid detection of virulence factor produced by Pseudomonas aeruginosacitations
- 2017Helbredstjek af dansk sundhedsteknologi
- 2017In-situ doped junctionless polysilicon nanowires field effect transistors for low-cost biosensorscitations
- 2015Fabrication and Characterisation of Membrane-Based Gold Electrodescitations
- 2014New approach of long-term modification of Topas® to acquire surface hydrophilicity for chromosome spreadingcitations
- 2013Doped Overoxidized Polypyrrole Microelectrodes as Sensors for the Detection of Dopamine Released from Cell Populationscitations
- 2013Doped Overoxidized Polypyrrole Microelectrodes as Sensors for the Detection of Dopamine Released from Cell Populationscitations
- 2013DNA hybridization sensing for cytogenetic analysis
- 2011Dielectrophoretic manipulation of human chromosomes in microfluidic channels: extracting chromosome dielectric propertiescitations
- 2010Conducting polymer 3D microelectrodescitations
- 2010Conducting polymer 3D microelectrodescitations
- 2009Metallization of high aspect ratio, out of plane structurescitations
Places of action
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document
DNA hybridization sensing for cytogenetic analysis
Abstract
Cytogenetic analysis focuses on studying the cell structure, mainly in respect to chromosome content and their structure. Chromosome abnormalities, such as translocations may cause various genetic disorders, but are also associated with heametological malignancies. Chromosome translocations are rearrangements between two chromosome arms that results in two derivative chromosomes having a mixed DNA sequence. The current detection method is a Fluorescent In situ Hybridization, which requires a use of expensive, fluorescently labeled probes that target the DNA sequences of two chromosomes involved in the translocation (Kwasny et al., 2012).<br/>We have developed a new double hybridization assay that allows for sorting of the DNA chromosomal fragments into separate compartment, moreover allowing for detection of the translocation. To detect the translocation it is necessary to determine that the two DNA sequences forming a derivative chromosome are connected, which is achieved by two subsequent hybridization steps. The first example of the translocation detection was presented on lab-on-a-disc using fluorescently labeled DNA fragments, representing the derivative chromosome (Brøgger et al., 2012). To allow for cheaper detection a label-free approach has been investigated using electrochemical impedance spectroscopy as a sensing method. We present here our recent results in regards to DNA sensing on metallic and conductive polymer electrodes for translocation detection. Our sensors are inexpensive and can be successfully applied in cytogenetic analysis as a replacement of standard techniques.<br/>