| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Cloetens, Peter
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (24/24 displayed)
- 2024Hard X-ray nanoscale Imaging of Carbon Fibre Composites using Near-Field Ptychography
- 2024Hard X-ray nanoscale Imaging of Carbon Fibre Composites using Near-Field Ptychography
- 2023Quantifying electrochemical degradation in single-crystalline LiNi0.8Mn0.1Co0.1O2–graphite pouch cells through operando X-ray and post-mortem investigations
- 2023Numerical microstructural optimization for the hydrogen electrode of solid oxide cellscitations
- 2022A stochastic geometrical 3D model for time evolution simulation of microstructures in SOC-electrodescitations
- 2020Ultrafine eutectic Ti-Fe-based alloys processed by additive manufacturing – A new candidate for high temperature applicationscitations
- 2020Ultrafine eutectic Ti-Fe-based alloys processed by additive manufacturing – A new candidate for high temperature applicationscitations
- 2019Nanovoid morphology and distribution in deformed High Density PolyEthylene observed at the nanometric scale
- 2017Inducing Stable $alpha$ + $beta$ Microstructures during Selective Laser Melting of Ti-6Al-4V Using Intensified Intrinsic Heat Treatmentscitations
- 2017Using metastability to engineer the microstructure of Ti-6V-4Al produced by selective laser melting
- 2017Inducing Stable alpha plus beta Microstructures during Selective Laser Melting of Ti-6Al-4V Using Intensified Intrinsic Heat Treatmentscitations
- 2015Three-dimensional characterization of fatigue-relevant intermetallic particles in high-strength aluminium alloys using synchrotron X-ray nanotomographycitations
- 2014Quantitative microstructure characterization of a Ni-YSZ bi-layer coupled with simulated electrode polarizationcitations
- 2014Anisotropic elasticity of silicon and its application to the modelling of X-ray opticscitations
- 2012Non-destructive 3-D reconstruction of the martensitic phase in a dual-phase steel using synchrotron holotomographycitations
- 2011The effect of the connectivity of rigid phases on strength of Al-Si alloyscitations
- 2009Polycaprolactone nanomesh cultured with hMSC evaluated by synchrotron tomography
- 2009New opportunities for 3D materials science of polycrystalline materials at the micrometre lengthscale by combined use of X-ray diffraction and X-ray imagingcitations
- 2009Projection phase contrast microscopy with a hard x-ray nanofocused beam: Defocus and contrast transfercitations
- 20093D architecture and load partition in eutectic Al-Si alloyscitations
- 2008In Situ X-Ray tomography studies of microstructural evolution combined with 3D modellingcitations
- 2006Advances in synchrotron radiation microtomographycitations
- 2005Permeability assessment by 3D interdendritic flow simulations on microtomography mappings of Al–Cu alloyscitations
- 2005Three dimensional imaging of damage in structural materials using high resolution micro-tomographycitations
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document
Polycaprolactone nanomesh cultured with hMSC evaluated by synchrotron tomography
Abstract
<p class="MsoNormal">Introduction</p><p><span style="font-size: 9.5pt; font-family: Times-Roman">Cell response is closely related to substrate stiffness. </span><span style="font-size: 10pt; font-family: 'Times New Roman'">Successful induced tissue repair from bioengineered constructs must possess both optimal bioactivity and mechanical strength. This is because cell interaction with the extracellular matrix (ECM) produces two different but concurrent signaling mechanisms: ligation-induced signaling, which depends on ECM biological stimuli, and traction-induced signaling, which depends on ECM mechanical stimuli, [1]. Different substrate stiffness </span><span style="font-size: 10pt; font-family: 'Times New Roman'">have</span><span style="font-size: 8pt; font-family: 'Times New Roman'"> </span><span style="font-size: 10pt; font-family: 'Times New Roman'">contrasting effects on migration and proliferation, where cells migrate faster on softer substrates while proliferating preferentially on the stiffer ones. This implicates that substrate rigidity is a critical design parameter in the development of scaffolds aimed at eliciting maximal cell and tissue function. From mechanics it is known that the stiffness of a porous structures scales with the relative density of the porous material, [2]. Hence, variations of substrate rigidity can be controlled through changes in relative density of the substrate itself. In three dimensional porous scaffolds, the substrate is equivalent to struts or beams randomly orientated in space making an interconnected network. These beams are called Plateau borders and are typically solid structures. Thus their stiffness depends solely on the stiffness of the selected biopolymer and the method of production. In this study we demonstrate that it is possible to control the porosity not only of the macroscopic porous scaffold but also of the Plateau borders constituting the scaffold.</span></p><p> </p><p class="MsoNormal" style="text-align: justify">Materials and Methods</p><p class="MsoNormal" style="text-align: justify">Polycaprolactone scaffolds were prepared by thermal induced phase separation followed by lyophilization. Processing conditions were chosen to range the relative density of the obtained scaffolds and its Plateau borders. Naked scaffolds and scaffolds cultivated statically with human bone marrow stromal cells, [3], for 24 hours, 14 days, and 21 days and prepared for holo-tomography.</p><p class="MsoNormal" style="text-align: justify">Synchrotron generated hard X-rays were used to perform quantitative phase sensitive holo-tomography at the ID19 beamline to obtain three-dimensional images of the processed and cultivated scaffolds, [4].</p><p class="MsoNormal" style="text-align: justify"></p><p class="MsoNoSpacing">Results and Discussion</p><p class="Columntext" style="margin-top: 4pt; margin-right: 0cm; margin-left: 0cm; margin-bottom: 0.0001pt; line-height: normal"><span style="font-size: 10pt">We have demonstrated that a double graded microstructure can be synthesised in this polycaprolactone system. It is possible to obtain specimens with solid Plateau borders, intermediate structures as shown in the figure and fully inversed microstructures in which the Plateau borders is demished and converted into a three dimensional nano sized mesh. <span class="Apple-style-span" style="font-family: 'Times New Roman', Arial, Helvetica, sans-serif">Results from specimens containing human stem cells show the attachement of cells to Plateau borders for the specimens cultivated for 24 hours. Specimens cultivated for 2 and 3 weeks shown the formations of extracellular matrix. </span></span></p><p class="MsoNormal" style="text-align: justify"></p><p class="MsoNormal">Conclusions</p><p class="MsoNormal" style="text-align: justify"><span style="font-size: 10pt; font-family: 'Times New Roman'">We have demonstrated that it is possible to control the microstructure of polycaprolactone based scaffolds. Microstructures can evolve into single and double graded structures, but also three dimensional fibrous nano meshes is realized. The morphology of the scaffold with and without human stem cells was investigated using tomography and numerical models were prepared for micromechanical modeling of cell scaffold interaction.<span class="Apple-style-span" style="font-family: Tahoma, Arial, Helvetica, sans-serif"> </span></span></p>