• Sitarek, Przemysław
• Zielińska-Bliźniewska, Hanna
• Olszewski, Jurek
• Majsterek, Ireneusz
• Miłoński, Jarosław
• Przybyłowska, Karolina

Abstract

Nasal polyps are strongly associated with a risk of chronicrhinosinusitis development as well as other obstruction in- cluding asthma and allergy. The following study tested theassociation of the 140A/G polymorphism of lactoferine(LF) encoding gene and the -33C/G polymorphism ofosteoblast-specific factor-2 (OSF-2) encoding gene with arisk of chronic rhinosinusitis with nasal polyps in a Polishpopulation. One hundred fifty eight patients of chronicrhinosinusitis with nasal polyps as well as 200 sex, age andethnicity matched control subjects without chronic sinusitisand nasal polyps were enrolled in this study. Among thegroup of patients 48 subjects were diagnosed with allergyand 50 subjects with asthma, respectively. DNA was iso- lated from peripheral blood lymphocytes of patients as wellas controls and gene polymorphisms were analyzed by re- striction fragments length polymorphism polymerase chainreaction (RFLP-PCR). We reported that the 140A/G LF(OR 4.56; 95% CI 2.87–7.26), the -33C/G OSF-2 OR 3.40;95% CI 2.08–5.57) and the -33G/G OSF-2 (OR 19.15;95% CI 7.72–47.52) genotypes were associated with anincreased risk of chronic rhinosinusitis with nasal polypsamong analyzed group of patients. Moreover, the groupof patients without allergy or asthma indicated the associa- tion of the -33C/G (OR 3.88; 95% CI 2.25–6.69 and OR3.83; 95% CI 2.25–6.69) and -33G/G (OR 17.8; 95% CI6.87–46.4 and OR 16.5; 95% CI 6.3–43.2) genotypes of theOSF-2 as wells as 140A/G (OR 4.10; 95% CI 2.47–6.81 andOR 4.13; 95% CI 2.47–6.88) genotype of OSF-2 with anincreased risk of chronic rhinosinusitis with nasal polyps.Finally, it was also found that the selected group of patientswith allergy or asthma indicated a very strong associationof the -33C/G (OR 3.12; 95% CI 1.45–6.73 and OR 3.12;95% CI 1.45–6.73, respectively) and -33G/G (OR 27.5;95% CI 9.56–79.4 and OR 24.5 ; 95% CI 8.30–71.4, respec- tively) genotypes of the OSF-2 as wells as 140A/G (OR4.86; 95% CI 2.38–9.95 and OR 4.74; 95% CI 2.35–9.55,respectively) genotypes with an increased risk of chronicrhinosinusitis with nasal polyps. Thus, our results suggestthat LF and OSF-2 gene polymorphisms may have deepimpact on the risk of rhinosinusitis nasal polyps’ formationwhich may also depend on asthma or allergy. Our resultsshowed that the 140A/G polymorphism of LF gene andthe -33C/G polymorphism of the OSF-2 gene may be associated with the risk of chronic rhinosinusitis with nasalpolyps in a Polish population.

Topics

• chemical ionisation