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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Sabac, Andrei
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Publications (7/7 displayed)
- 2022A membrane-less Glucose/O2 non-enzymatic fuel cell based on bimetallic Pd–Au nanostructure anode and air-breathing cathode: Towards micro-power applications at neutral pHcitations
- 2022Cedar Wood-Based Biochar: Properties, Characterization, and Applications as Anodes in Microbial Fuel Cellcitations
- 2017Minimally invasive microelectrode biosensors reveal different neurochemical signature of spreading depolarization in rat cortex.
- 2017Platinized Carbon Fibers as an Electrochemical Substrate to Obtain Minimally Invasive Microelectrode Biosensors for Brain Monitoring
- 2005Nanostructuring lithium niobate substrates by focused ion beam millingcitations
- 2004Evaluation of the mechanical properties of square membranes prestressed by PECVD silicon oxynitride thin filmscitations
- 2004Optomechanical characterisation of compressively prestressed silicon oxynitride films deposited by plasma-enhanced chemical vapour deposition on silicon membranescitations
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document
Minimally invasive microelectrode biosensors reveal different neurochemical signature of spreading depolarization in rat cortex.
Abstract
Monitoring the chemical composition of the brain interstitial fluid is an important challenge for both pre-clinical and clinical research on brain injury. Microelectrode biosensors are a promising technique with a temporal resolution in the order ofseconds. Here, ultra-microelectrodes based on platinized carbon fibers were fabricated to obtain biosensors with less than 15 µm external diameter. Platinization was achieved by sputtering a 10 nm Cr adhesion layer followed by 100 nm of platinum. Platinized carbon fibers were then encased in a glass micropipette and covered with electropolymerized poly-phenylenediamine for selectivity, and covalently immobilized oxidase enzymes (glucose oxidase, lactate oxidase, D-amino acid oxidase or glutamate oxidase). After implantation in the rat parietal cortex, such biosensors detected a smaller basal lactate concentration and a slower diffusion of glucose and D-serine through the blood brain barrier compared to more conventional biosensors with 100 µm external diameter. Interestingly, spreading depolarization (SD) produced a smaller increase in lactate, a larger decrease in glucose, and a larger increase in D-serine at platinized carbon fibers microelectrode biosensors compared to larger sensors. Therefore, the neurochemical signature of SDs was significantly different when estimated with these new minimally invasive biosensors. Such small devices avoid major mechanical injury to blood vessels, preserve the blood brain barrier at the site of implantation, and therefore, provide more accurate measurements from the brain interstitial fluid. Developing smaller, less invasive probes for brain monitoring is therefore an important challenge in order to obtain meaningful information about the cellular mechanisms at work during brain injury.