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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Moura, José J. G.
Universidade Nova de Lisboa
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (14/14 displayed)
- 2019Biosensor for direct bioelectrocatalysis detection of nitric oxide using nitric oxide reductase incorporated in carboxylated single-walled carbon nanotubes/lipidic 3 bilayer nanocompositecitations
- 2019Biosensor for direct bioelectrocatalysis detection of nitric oxide using nitric oxide reductase incorporated in carboxylated single-walled carbon nanotubes/lipidic 3 bilayer nanocompositecitations
- 2019Third-generation electrochemical biosensor based on nitric oxide reductase immobilized in a multiwalled carbon nanotubes/1-n-butyl-3-methylimidazolium tetrafluoroborate nanocomposite for nitric oxide detectioncitations
- 2014Influence of respiratory substrate in carbon steel corrosion by a Sulphate Reducing Prokaryote model organismcitations
- 2011Crystal structure of the zinc-, cobalt-, and iron-containing adenylate kinase from Desulfovibrio gigas: a novel metal-containing adenylate kinase from Gram-negative bacteria
- 2011Crystal structure of the zinc-, cobalt-, and iron-containing adenylate kinase from Desulfovibrio gigas: a novel metal-containing adenylate kinase from Gram-negative bacteriacitations
- 2011Bacterial diversity and influence of SRB presence on metal behaviour within the oil & gas industry
- 2009Isolation and characterization of a new Cu-Fe protein from Desulfovibrio aminophilus DSM12254citations
- 2009Cobalt-, zinc- and iron-bound forms of adenylate kinase (AK) from the sulfate-reducing bacterium Desulfovibrio gigas: purification, crystallization and preliminary X-ray diffraction analysiscitations
- 2005Superoxide reductase from the syphilis spirochete Treponema pallidum: crystallization and structure determination using soft X-rayscitations
- 2004Overexpression and purification of Treponema pallidum rubredoxin; kinetic evidence for a superoxide-mediated electron transfer with the superoxide reductase neelaredoxincitations
- 2003Formation of a stable cyano-bridged dinuclear iron cluster following oxidation of the superoxide reductases from treponema pallidum and Desulfovibrio vulgaris with K3Fe(CN)6citations
- 2001Tungsten-containing formate dehydrogenase from Desulfovibrio gigas: metal identification and preliminary structural data by multi-wavelength crystallographycitations
- 2000Evidence for antisymmetric exchange in cuboidal [3Fe-4S]+ clusterscitations
Places of action
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document
Bacterial diversity and influence of SRB presence on metal behaviour within the oil & gas industry
Abstract
<p>Biocorrosion concerns especially the Oil and Gas (O&G) industry, where it leads to extensive and costly damages for the equipment and consequent production losses. Sulfate Reducing Bacteria (SRB) are an important group of microorganisms involved in the biocorrosion process. In this work we aim to analyze microbial diversity and the electrochemical behaviour of metals from offshore installations, using a model SRB organism. Four bio-coupons were placed in anaerobic bottles with VMN Sulfate medium (VMN-S) flushed with argon and incubated at 37°C and 30°C for a period of 48h to 2 weeks. The samples presented a limited growth and were used as inoculums for a second incubation in VMN Nitrate medium (VMN-N), under the same conditions. Cells were harvested and used for DNA extraction followed by PCR and DGGE analysis. After this, the bio-coupons were prepared to be used as working electrodes in the presence of Desulfovibrio desulfuricans 27774. The Open Circuit Potential (OCP) was measured over 6 days using VMN-S as electrolyte. Four ecotypes were observed in the DGGE gel and are being prepared for DNA sequencing. The OCP showed a variation around 200mV when both conditions were compared. The increase of potential is concomitant to the appearance of a stationary phase and could be related to biofilm establishment or normal metabolism (as sulfides production). Further analyses are in progress to confirm the role of bacterial attachment in the metal behaviour. Our study gives new proofs of the growth phase influence within the biocorrosion evolution in carbon steel used by the O&G industry.</p>