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Zillen, Daan
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document
Stereolithographic fabrication and characterization of immobilized enzyme reactors for in vitro digestions
Abstract
Enzymatic digestion is a key process in the gastrointestinal tract that we recreated in an earlier miniaturized system. In order to avoid the need to continuously supply digestive enzymes, this work studied the immobilization of digestive enzymes in a microsystem. Flow-through reactors were produced by stereolithography and the digestive enzyme, alpha-amylase, was immobilized onto the channel walls. The activity of immobilized alpha-amylase was determined by digestion of starch, with potential applications in organs-on-a-chip or other enzymatic conversions.