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Ioannidis, Nicolas
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Publications (3/3 displayed)
- 2012Manufacturing of agarose-based chromatographic adsorbents – effect of ionic strength and cooling conditions on particle structure and mechanical strengthcitations
- 2012Manufacturing of agarose-based chromatographic adsorbents with controlled pore and particle sizes
- 2011Manufacturing of agarose-based chromatographic adsorbents with controlled pore and particle size
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document
Manufacturing of agarose-based chromatographic adsorbents with controlled pore and particle sizes
Abstract
<p>The effect of ionic strength and quenching temperature on the mechanical properties and structure of agarose-based chromatographic adsorbents in micro-beads for bioseparation was investigated. The pore size/size distribution of the beads was measured by analysis of their AFM images. Their mechanical properties were determined by a micromanipulation technique based on compression of single micro-particles. It was found that the mean pore size and stiffness of agarose beads increase with ionic strength and slow cooling. These two parameters do not affect the particle size/size distribution.</p>