| People | Locations | Statistics |
|---|---|---|
| Naji, M. |
| |
| Motta, Antonella |
| |
| Aletan, Dirar |
| |
| Mohamed, Tarek |
| |
| Ertürk, Emre |
| |
| Taccardi, Nicola |
| |
| Kononenko, Denys |
| |
| Petrov, R. H. | Madrid |
|
| Alshaaer, Mazen | Brussels |
|
| Bih, L. |
| |
| Casati, R. |
| |
| Muller, Hermance |
| |
| Kočí, Jan | Prague |
|
| Šuljagić, Marija |
| |
| Kalteremidou, Kalliopi-Artemi | Brussels |
|
| Azam, Siraj |
| |
| Ospanova, Alyiya |
| |
| Blanpain, Bart |
| |
| Ali, M. A. |
| |
| Popa, V. |
| |
| Rančić, M. |
| |
| Ollier, Nadège |
| |
| Azevedo, Nuno Monteiro |
| |
| Landes, Michael |
| |
| Rignanese, Gian-Marco |
|
Djearamane, Sinouvassane
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (3/3 displayed)
- 2024Innovative use of chitosan/ZnO NPs bio-nanocomposites for sustainable antimicrobial food packaging of poultry meat
- 2023Tribological characterization of sponge gourd outer skin fiber-reinforced epoxy composite with <i>Tamarindus indica</i> seed filler addition using the Box–Behnken methodcitations
- 2019Cellular accumulation and cytotoxic effects of zinc oxide nanoparticles in microalga Haematococcus pluvialiscitations
Places of action
| Organizations | Location | People |
|---|
article
Cellular accumulation and cytotoxic effects of zinc oxide nanoparticles in microalga Haematococcus pluvialis
Abstract
<jats:sec> <jats:title>Background</jats:title> <jats:p>Zinc oxide nanoparticles (ZnO NPs) are widely used in household and cosmetic products which imply an increased releasing of these particles into the environment, especially aquatic ecosystems, resulting in the need of assessing the potential toxic effects of ZnO NPS on the aquatic organisms, particularly on microalgae which form the base for food chain of aquatic biota. The present study has investigated the dose- and time-dependent cellular accumulation and the corresponding cytotoxic effects of increasing concentrations of ZnO NPs from 10–200 μg/mL on microalga <jats:italic>Haematococcus pluvialis</jats:italic> at an interval of 24 h for 96 h.</jats:p> </jats:sec> <jats:sec> <jats:title>Methods</jats:title> <jats:p>The scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX) was used to qualitatively detect the cellular accumulation of ZnO NPs in algal cells, while inductively coupled plasma optical emission spectrometry (ICP OES) was performed to quantify the cell associated-zinc in algal cells. The percentage of cell death, reduction in algal biomass, and loss in photosynthetic pigments were measured to investigate the cytotoxic effects of ZnO NPs on <jats:italic>H. pluvialis</jats:italic>. Extracellular and intracellular changes in algal cells resulted from the treatment of ZnO NPs were demonstrated through optical, scanning, and transmission electron microscopic studies.</jats:p> </jats:sec> <jats:sec> <jats:title>Results</jats:title> <jats:p>SEM-EDX spectrum evidenced the accumulation of ZnO NPs in algal biomass and ICP OES results reported a significant (<jats:italic>p</jats:italic> < 0.05) dose- and time-dependent accumulation of zinc in algal cells from 24 h for all the tested concentrations of ZnO NPs (10–200 μg/mL). Further, the study showed a significant (<jats:italic>p</jats:italic> < 0.05) dose- and time-dependent growth inhibition of <jats:italic>H. pluvialis</jats:italic> from 72 h at 10–200 μg/mL of ZnO NPs. The morphological examinations revealed substantial surface and intracellular damages in algal cells due to the treatment of ZnO NPs.</jats:p> </jats:sec> <jats:sec> <jats:title>Discussion</jats:title> <jats:p>The present study reported the significant cellular accumulation of ZnO NPs in algal cells and the corresponding cytotoxic effects of ZnO NPs on <jats:italic>H. pluvialis</jats:italic> through the considerable reduction in algal cell viability, biomass, and photosynthetic pigments together with surface and intracellular damages.</jats:p> </jats:sec>