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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Martel, Anne
Institut Laue-Langevin
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (12/12 displayed)
- 2022Mg2+-dependent conformational equilibria in CorA and an integrated view on transport regulationcitations
- 2022Mg2+-dependent conformational equilibria in CorA and an integrated view on transport regulationcitations
- 2022Small-angle x-ray and neutron scattering of MexR and its complex with DNA supports a conformational selection binding model.citations
- 2022A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarkingcitations
- 2022A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarkingcitations
- 2021Interpenetrated biosurfactant-silk fibroin networks – a SANS studycitations
- 2021Pepsi-SAXS/SANS -small-angle scattering-guided tools for integrative structural bioinformaticscitations
- 2021Mg2+-dependent conformational equilibria in CorA: an integrated view on transport regulation
- 2020Hemicellulose binding and the spacing of cellulose microfibrils in spruce woodcitations
- 2017Fast Collisional Lipid Transfer Among Polymer-Bounded Nanodiscs.citations
- 2016Dimeric peptides with three different linkers self-assemble with phospholipids to form peptide nanodiscs that stabilize membrane proteinscitations
- 2015Large-Scale Conformational Dynamics Control H5N1 Influenza Polymerase PB2 Binding to Importin α.citations
Places of action
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article
Mg2+-dependent conformational equilibria in CorA and an integrated view on transport regulation
Abstract
<p>The CorA family of proteins regulates the homeostasis of divalent metal ions in many bacteria, archaea, and eukaryotic mitochondria, making it an important target in the investigation of the mechanisms of transport and its functional regulation. Although numerous structures of open and closed channels are now available for the CorA family, the mechanism of the transport regulation remains elusive. Here, we investigated the conformational distribution and associated dynamic behaviour of the pentameric Mg2+ channel CorA at room temperature using small-angle neutron scattering (SANS) in combination with molecular dynamics (MD) simulations and solid-state nuclear magnetic resonance spectroscopy (NMR). We find that neither the Mg2+-bound closed structure nor the Mg2+-free open forms are sufficient to explain the average conformation of CorA. Our data support the presence of conformational equilibria between multiple states, and we further find a variation in the behaviour of the backbone dynamics with and without Mg2+. We propose that CorA must be in a dynamic equilibrium between different non-conducting states, both symmetric and asymmetric, regardless of bound Mg2+ but that conducting states become more populated in Mg2+-free conditions. These properties are regulated by backbone dynamics and are key to understanding the functional regulation of CorA.</p>