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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Yu, X.
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (11/11 displayed)
- 2024High temperature co-polyester thermoplastic elastomer nanocomposites for flexible self-regulating heating devicescitations
- 2024Temperature and structure measurements of heavy-ion-heated diamond using in situ X-ray diagnosticscitations
- 2023On the importance of Crystal Plasticity Finite Element discretisation for the identification of crack initiation in RCF using energy-based criteriacitations
- 2020Quasi-quantitative photo-conversion of methane to ethane at ambient temperature on silver based nanocomposites
- 2020Luminescence behavior of semipolar (101¯1) InGaN/GaN “bow-tie” structures on patterned Si substrates
- 2020Stoichiometric methane conversion to ethane using photochemical looping at ambient temperaturecitations
- 2018Micro-tribological performance of fullerene-like carbon and carbon-nitride surfacescitations
- 2016IR-spectroscopy of CO adsorption on mixed-terminated ZnO surfacescitations
- 2015Cis-to-trans isomerization of azobenzene investigated by using thin films of metal-organic frameworkscitations
- 2014Perfluoroalkyl-Functionalized Thiazole–Thiophene Oligomers as N-Channel Semiconductors in Organic Field-Effect and Light-Emitting Transistorscitations
- 2013ANXA2 regulates the behavior of SGC-7901 cells.citations
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article
ANXA2 regulates the behavior of SGC-7901 cells.
Abstract
ANXA2, a member of the annexin family, is overexpressed and plays important roles in tumor development. However, the significance of ANXA2 expression in gastric carcinoma has not been clarified.To elucidate its roles in growth of gastric cancer, ANXA2 expression in SGC-7901 cells was inhibited with a designated siRNA, then cell proliferation, cell cycling, apoptosis and motility were determined by MTT assay, flow cytometry, Hoechst 33342 staining and wound healing assay, respectively. To further assess the behavior of ANXA2 deleted SGC- 7901 cells, changes of microstructures were observed under fluorescence microscopy, laser scanning confocal microscopy and electron microscopy. We found that inhibition of ANXA2 expression caused cell proliferation to decrease significantly with G1 arrest, motility to be reduced with changes in pseudopodia/filopodia structure and F-actin and β-tubulin expression, and apoptosis to be enhanced albeit without significance. At the same time, ANXA2 deletion resulted in fewer pseudopodia/filopodia, non-stained areas were increased, contact inhibition among cells reappeared, and expression of F-actin and β-tubulin was decreased, with induction of polymerized disassembled forms. Taken together, these data suggest that ANXA2 overexpression is important to maintain the malignancy of cancer cells, and this member of the annexin family has potential to be considered as a target for the gene therapy of gastric carcinoma.