• Almaroof, Ahmed
• Banerjee, Avijit
• Mannocci, Francesco
• Festy, Frederic
• Ali, Ahmed

Abstract

<p>Aim: This study aimed to evaluate the mineral deposition and microhardness within the retained caries-affected dentin (CAD) after excavation with Carisolv™ gel or rotary burs. Materials and methods: Totally 20 extracted human carious molars, with caries extending into the middle third of dentin, were sectioned mesiodistally. The carious tissue in each hemi-section was excavated with gel or bur and restored with a mineral trioxide aggregate (MTA) base and resin composite. Five sound posterior teeth were hemi-sected to serve as a control. Twenty-five hemi-sections were stored in distilled water (DW) and the other 25 were stored in simulated body fluid (SBF). Each sample was scanned and tested using Raman spectroscopy and Knoop microhardness respectively, at the MTA-dentin interface at day 1 and 14. Scanning electron microscope (SEM) was used to assess the surface topography of dentin after excavation with gel/bur. Results: The results show that the baseline mineral content and microhardness of dentin were significantly lower in Carisolv™-treated samples in comparison with that treated with burs (p ≤ 0.05). However, there were comparable mineral levels in the two groups after 14 days’ storage in SBF (p ≥ 0.05), which showed statistically insignificant differences from the sound control (p ≥ 0.05). Microhardness and mineral contents decreased significantly in the sound control after 14 days’ storage in DW (p ≤ 0.05). The SEM images showed partially open dentin tubules with less smear layer after Carisolv™ excavation compared with more occluded dentin tubules with an abundance of smear layer after bur excavation. Conclusion: Although gel excavation retains more CAD in comparison with bur, remineralization of this remaining tissue is evident after 2 weeks. Clinical significance: The use of Carisolv™ gel provides an alternative to rotary burs in terms of preserving the tooth structure and not hindering the remineralization potential of valuable tooth structure, a clinical advantage for minimally invasive dentistry.</p>

Topics

• Deposition
• impedance spectroscopy
• mineral
• surface
• scanning electron microscopy
• laser emission spectroscopy
• composite
• resin
• Raman spectroscopy
• collision-induced dissociation