| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Estrela, Pedro
University of Bath
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (17/17 displayed)
- 2021CRISPR-based electrochemical sensor for COVID-19 diagnostics
- 2021Graphene enabled low-noise surface chemistry for multiplexed sepsis biomarker detection in whole bloodcitations
- 2020Electrochemical ELISA Protein Biosensing in Undiluted Serum Using a Polypyrrole-Based Platformcitations
- 2019A PNA-based Lab-on-PCB diagnostic platform for rapid and high sensitivity DNA quantificationcitations
- 2019Reduced Graphene-Oxide Transducers for Biosensing Applications Beyond the Debye-Screening Limitcitations
- 2019In-situ synthesis of 3D ultra-small gold augmented graphene hybrid for highly sensitive electrochemical binding capabilitycitations
- 2019Multiplexed Electrochemical Platform for sepsis Diagnostics
- 2017Raman and Mössbauer spectroscopic studies of tungsten doped Ni–Zn nano ferritecitations
- 2017Nanomaterial fungicides: In vitro and in vivo antimycotic activity of cobalt and nickel nanoferrites on phytopathogenic fungicitations
- 2016Electrochemical biosensors and nanobiosensorscitations
- 2016Inexpensive and fast pathogenic bacteria screening using field-effect transistorscitations
- 2016Inexpensive and fast pathogenic bacteria screening using field-effect transistorscitations
- 2016Aptamer-MIP hybrid receptor for highly sensitive electrochemical detection of prostate specific antigencitations
- 2011Room temperature processed ISFETs based on amorphous semiconductors oxides
- 2002High pressure transport study of non-Fermi liquid behaviour in U2Pt2In and U3Ni3Sn4
- 2002Pressure-induced recovery of the Fermi-liquid state in the non-Fermi liquid material U2Pt2Incitations
- 2000Possible non-Fermi-liquid behaviour in URh1/3Ni2/3Alcitations
Places of action
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article
Electrochemical ELISA Protein Biosensing in Undiluted Serum Using a Polypyrrole-Based Platform
Abstract
An electrochemical enzyme-linked immunosorbent assay (ELISA) biosensor platform using electrochemically prepared ~11 nm thick carboxylic functionalized popypyrrole film has been developed for bio-analyte measurement in undiluted serum. Carboxyl polypyrrole (PPy-COOH) film using 3-carboxy-pyrrol monomer onto comb-shaped gold electrode microarray (Au) was prepared via cyclic voltammetry (CV). The prepared Au/PPy-COOH was then utilized for electrochemical ELISA platform development by immobilizing analyte-specific antibodies. Tumor necrosis factor-alpha (TNF-α) was selected as a model analyte and detected in undiluted serum. For enhanced performance, the use of a polymeric alkaline phosphatase tag was investigated for the electrochemical ELISA. The developed platform was characterized at each step of fabrication using CV, electrochemical impedance spectroscopy and atomic force microscopy. The bioelectrodes exhibited linearity for TNF-α in the 100 pg/mL–100 ng/mL range when measured in spiked serum, with limit of detection of 78 pg/mL. The sensor showed insignificant signal disturbance from serum proteins and other biologically important proteins. The developed platform was found to be fast and specific and can be applicable for testing and measuring various biologically important protein markers in real samples.