693.932 PEOPLE
| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Del Valle, Manel
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Topics
Publications (37/37 displayed)
- 2021Wavelet neural networks
- 2020Improved Sensing of Capsaicin with TiO2 Nanoparticles Modified Epoxy Graphite Electrode
- 2018Bioelectronic tongue using MIP sensors for the resolution of volatile phenolic compounds
- 2018Multivariate Determination of Total Sugar Content and Ethanol in Bioethanol Production Using Carbon Electrodes Modified with MWCNT/MeOOH and Chemometric Data Treatment
- 2018Label-free aptasensor for lysozyme detection using electrochemical impedance spectroscopycitations
- 2017Molecularly imprinted polymers for TNT analogues :citations
- 2017Graphene electrode platform for impedimetric aptasensing
- 2016Analysis of Amino Acid Mixtures by Voltammetric Electronic Tongues and Artificial Neural Networks
- 2016Simultaneous Voltammetric Determination of Heavy Metals by Use of Crown Ether-modified Electrodes and Chemometricscitations
- 2016Resolution of galactose, glucose, xylose and mannose in sugarcane bagasse employing a voltammetric electronic tongue formed by metals oxy-hydroxide/MWCNT modified electrodescitations
- 2015Crown ether-modified electrodes for the simultaneous stripping voltammetric determination of Cd(II), Pb(II) and Cu(II)citations
- 2015DNA polymorphism sensitive impedimetric detection on gold-nanoislands modified electrodescitations
- 2014Discrimination of Soils and Assessment of Soil Fertility Using Information from an Ion Selective Electrodes Array and Artificial Neural Networkscitations
- 2014Label-free impedimetric aptasensor based on epoxy-graphite electrode for the recognition of cytochrome c
- 2014A comparison of four protocols for the immobilization of an aptamer on graphite composite electrodes
- 2014Array of peptide-modified electrodes for the simultaneous determination of Pb(II), Cd(II) and Zn(II)
- 2014Pd nanoparticles/multiwalled carbon nanotubes electrode system for voltammetric sensing of tyrosinecitations
- 2014Impedimetric DNA Biosensors Based on Nanomaterialscitations
- 2012Determination of total polyphenol index in wines employing a voltammetric electronic tonguecitations
- 2012Impedimetric DNA Sensing Employing Nanomaterialscitations
- 2012A reusable impedimetric aptasensor for detection of thrombin employing a graphite-epoxy composite electrodecitations
- 2011Multiway processing of data generated with a potentiometric electronic tongue in a SIA systemcitations
- 2011A new amperometric bienzymatic biosensor based on biocomposites for the determination of gluconic acid in winescitations
- 2010Use of Sequential Injection Analysis to construct a potentiometric electronic tongue: Application to the multidetermination of heavy metalscitations
- 2009Electrochemical immunosensor for the diagnosis of celiac diseasecitations
- 2009Resolution of heavy metal mixtures from highly overlapped ASV voltammograms employing a wavelet neural networkcitations
- 2007EIS multianalyte sensing with an automated SIA system-An electronic tongue employing the impedimetric signalcitations
- 2007Application of the avidin-biotin interaction to immobilize DNA in the development of electrochemical impedance genosensorscitations
- 2007Multicomponent titration of calcium + magnesium mixtures employing a potentiometric electronic-tonguecitations
- 2006Impedimetric genosensors for the detection of DNA hybridizationcitations
- 2006Genomagnetic assay based on label-free electrochemical detection using magneto-composite electrodescitations
- 2006Urea impedimetric biosensor based on polymer degradation onto interdigitated electrodescitations
- 2005Integration of a glucose biosensor based on an epoxy-graphite- TTF·TCNQ-GOD biocomposite into a FIA systemcitations
- 2005Glucose biosensor based on carbon nanotube epoxy compositescitations
- 2005Sensitive stripping voltammetry of heavy metals by using a composite sensor based on a built-in bismuth precursorcitations
- 2004Renewable Protein A modified graphite-epoxy composite for electrochemical immunosensingcitations
- 2004Rigid carbon composites: A new transducing material for label-free electrochemical genosensingcitations
Places of action
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article
Label-free aptasensor for lysozyme detection using electrochemical impedance spectroscopy
Abstract
© 2018 by the authors.This research develops a label-free aptamer biosensor (aptasensor) based on graphite-epoxy composite electrodes (GECs) for the detection of lysozyme protein using Electrochemical Impedance Spectroscopy (EIS) technique. The chosen immobilization technique was based on covalent bonding using carbodiimide chemistry; for this purpose, carboxylic moieties were first generated on the graphite by electrochemical grafting. The detection was performed using [Fe(CN) 6 ] 3- /[Fe(CN) 6 ] 4- as redox probe. After recording the frequency response, values were fitted to its electric model using the principle of equivalent circuits. The aptasensor showed a linear response up to 5 µMfor lysozyme and a limit of detection of 1.67 µM. The sensitivity of the established method was 0.090 µM -1 in relative charge transfer resistance values. The interference response by main proteins, such as bovine serum albumin and cytochrome c, has been also characterized. To finally verify the performance of the developed aptasensor, it was applied to wine analysis.