• Liustrovaite, Viktorija
• Korniienko, Viktoriia
• Holubnycha, Viktoriia
• Ratautaite, Vilma
• Diedkova, Kateryna
• Pogorielov, Maksym
• Pilvenytė, Greta
• Ramanavicius, Arunas
• Bogužaitė, Raimonda
• Ramanaviciene, Almira
• Viter, Roman

Abstract

<jats:p>Detecting bacteria—Listeria monocytogenes—is an essential healthcare and food industry issue. The objective of the current study was to apply platinum (Pt) and screen-printed carbon (SPCE) electrodes modified by molecularly imprinted polymer (MIP) in the design of an electrochemical sensor for the detection of Listeria monocytogenes. A sequence of potential pulses was used to perform the electrochemical deposition of the non-imprinted polypyrrole (NIP-Ppy) layer and Listeria monocytogenes-imprinted polypyrrole (MIP-Ppy) layer over SPCE and Pt electrodes. The bacteria were removed by incubating Ppy-modified electrodes in different extraction solutions (sulphuric acid, acetic acid, L-lysine, and trypsin) to determine the most efficient solution for extraction and to obtain a more sensitive and repeatable design of the sensor. The performance of MIP-Ppy- and NIP-Ppy-modified electrodes was evaluated by pulsed amperometric detection (PAD). According to the results of this research, it can be assumed that the most effective MIP-Ppy/SPCE sensor can be designed by removing bacteria with the proteolytic enzyme trypsin. The LOD and LOQ of the MIP-Ppy/SPCE were 70 CFU/mL and 210 CFU/mL, respectively, with a linear range from 300 to 6700 CFU/mL.</jats:p>

Topics

• Deposition
• impedance spectroscopy
• polymer
• Carbon
• extraction
• Platinum