| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Larsen, Niels Bent
Technical University of Denmark
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (22/22 displayed)
- 2023Contrast-enhanced ultrasound imaging using capacitive micromachined ultrasonic transducerscitations
- 2022High Resolution Dual Material Stereolithography for Monolithic Microdevicescitations
- 2022Immobilization of Active Antibodies at Polymer Melt Surfaces during Injection Molding
- 20213D printed calibration micro-phantoms for super-resolution ultrasound imaging validationcitations
- 20193D Printed Calibration Micro-phantoms for Validation of Super-Resolution Ultrasound Imagingcitations
- 2015Hydrogen silsesquioxane mold coatings for improved replication of nanopatterns by injection moldingcitations
- 2013Injection molding of high aspect ratio sub-100 nm nanostructurescitations
- 2013Designing CAF-adjuvanted dry powder vaccinescitations
- 2012A Platform for Functional Conductive Polymers
- 2012Micropatterning of Functional Conductive Polymers with Multiple Surface Chemistries in Registercitations
- 2011Enhanced transduction of photonic crystal dye lasers for gas sensing via swelling polymer filmcitations
- 2011Injection molded nanofluidic chips: Fabrication method and functional tests using single-molecule DNA experimentscitations
- 2011Microwave assisted click chemistry on a conductive polymer filmcitations
- 2011Selective gas sensing for photonic crystal lasers
- 2010Fast prototyping of injection molded polymer microfluidic chipscitations
- 2010Nanostructures for all-polymer microfluidic systemscitations
- 2010“Electro-Click” on Conducting Polymer Films
- 2008Novel polymer coatings based on plasma polymerized 2-methoxyethyl acrylate
- 2008Conductive Polymer Functionalization by Click Chemistrycitations
- 2007Micropatterning of a stretchable conductive polymer using inkjet printing and agarose stampingcitations
- 2006On the Injection Molding of Nanostructured Polymer Surfacescitations
- 2001Surface morphology of PS-PDMS diblock copolymer filmscitations
Places of action
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article
Immobilization of Active Antibodies at Polymer Melt Surfaces during Injection Molding
Abstract
<p>We demonstrate the transfer and immobilization of active antibodies from a low surface- energy mold surface to thermoplastic replica surfaces using injection molding, and we investigate the process at molecular scale. The transfer process is highly efficient, as verified by atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS) of the mold and replica surfaces. AFM analysis reveals partial nanometer-scale embedding of the protein into the polymer matrix as a possible mechanism of permanent immobilization. Replicas with rabbit anti-mouse IgG immobilized as capture antibody at the hot polymer melt surface during injection molding show similar affinity for their antigen (mouse IgG) in sandwich enzyme-linked immunosorbent assay (ELISA) as capture antibodies deposited by passive adsorption onto a bare thermoplastic replica. The transferred antibodies retain their functionality after incubation in serum-containing cell medium for >1 week. A mold coating time of 10 min prior to injection molding is sufficient for producing highly sensitive ELISA assays, thus enabling the short processing cycle times required for mass production of single-use biodevices relying on active immobilized antibodies.</p>