• Hannula, Simo-Pekka
• Granbohm, Henrika
• Mosselhy, Dina
• Nordstrom, Katrina
• Palva, Airi
• Ge, Yanling
• Hynönen, Ulla Eeva Elina

Abstract

<p>Infected superficial wounds were traditionally controlled by topical antibiotics until the emergence of antibiotic-resistant bacteria. Silver (Ag) is a kernel for alternative antibacterial agents to fight this resistance quandary. The present study demonstrates a method for immobilizing small-sized (similar to 5 nm) silver nanoparticles on silica matrix to form a nanosilver-silica (Ag-SiO2) composite and shows the prolonged antibacterial effects of the composite in vitro. The composite exhibited a rapid initial Ag release after 24 h and a slower leaching after 48 and 72 h and was effective against both methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli). Ultraviolet (UV)-irradiation was superior to filter-sterilization in retaining the antibacterial effects of the composite, through the higher remaining Ag concentration. A gauze, impregnated with the Ag-SiO2 composite, showed higher antibacterial effects against MRSA and E. coli than a commercial Ag-containing dressing, indicating a potential for the management and infection control of superficial wounds. Transmission and scanning transmission electron microscope analyses of the composite-treated MRSA revealed an interaction of the released silver ions with the bacterial cytoplasmic constituents, causing ultimately the loss of bacterial membranes. The present results indicate that the Ag-SiO2 composite, with prolonged antibacterial effects, is a promising candidate for wound dressing applications.</p>

Topics

• nanoparticle
• impedance spectroscopy
• silver
• composite
• leaching