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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Wolff, Anders
Technical University of Denmark
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (14/14 displayed)
- 2019Fabrication of 3D microstructure array on chip for rapid pathogen detectioncitations
- 2019Large-scale fabrication of microfluidic chips with three-dimensional microstructures for point of care application
- 2019Large-scale fabrication of microfluidic chips with three-dimensional microstructures for point of care application
- 2019A Complete Protocol for Rapid and Low-Cost Fabrication of Polymer Microfluidic Chips Containing Three-Dimensional Microstructures Used in Point-of-Care Devicescitations
- 2016Laser ablated micropillar energy directors for ultrasonic welding of microfluidic systemscitations
- 2016MICRO-SCALE ENERGY DIRECTORS FOR ULTRASONIC WELDING
- 2015Ultrasonic welding for fast bonding of self-aligned structures in lab-on-a-chip systemscitations
- 2014Fabrication and modelling of injection moulded all-polymer capillary microvalves for passive microfluidic controlcitations
- 2012A novel detection platform for parallel monitoring of DNA hybridization with high sensitivity and specificity
- 2012A novel detection platform for parallel monitoring of DNA hybridization with high sensitivity and specificity
- 2010Microfluidic DNA microarrays in PMMA chips: streamlined fabrication via simultaneous DNA immobilization and bonding activation by brief UV exposurecitations
- 2007PCR biocompatibility of Lab-on-a-chip and MEMS materialscitations
- 2006Dielectrophoresis microsystem with integrated flow cytometers for on-line monitoring of sorting efficiencycitations
- 2004Numerical simulation of travelling wave induced electrothermal fluid flowcitations
Places of action
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article
A Complete Protocol for Rapid and Low-Cost Fabrication of Polymer Microfluidic Chips Containing Three-Dimensional Microstructures Used in Point-of-Care Devices
Abstract
This protocol provides insights into the rapid, low-cost, and largescale fabrication of polymer microfluidic chips containing three-dimensional microstructures used in point-of-care devices for applications such as detection of pathogens via molecular diagnostic methods. The details of the fabrication methods are described in this paper. This study offers suggestions for researchers and experimentalists, both at university laboratories and in industrial companies, to prevent doom fabrication issues. For a demonstration of bio-application in point-of-care testing, the 3D microarrays fabricated are then employed in multiplexed detection of Salmonella (Salmonella Typhimurium and Salmonella Enteritidis), based on a molecular detection technique called solid-phase polymerase chain reaction (SP-PCR).