• Cahill, Paul A.
• Mcivor, Mary Josephine
• Meenan, Brian
• Fishlock, Sam
• Mceneaney, David J.
• Forster, Robert
• Mcferran, Aoife
• Boyd, Adrian
• Acheson, Jonathan
• Maolmhuaidh, Fionn Ó.
• Meenagh, Aidan
• Ward, Joanna
• Hussain, Shahzad
• Bhattacharya, Gourav

Abstract

Polycaprolactone (PCL) is a well-established biomaterial, offering extensive mechanical attributes along with low cost, biocompatibility, and biodegradability; however, it lacks hydrophilicity, bioactivity, and electrical conductivity. Advances in 3D fabrication technologies allow for these sought-after attributes to be incorporated into the scaffolds during fabrication. In this study, solvent-free Fused Deposition Modelling was employed to fabricate 3D scaffolds from PCL with increasing amounts of graphene (G), in the concentrations of 0.75, 1.5, 3, and 6% (w/w). The PCL+G scaffolds created were characterised physico-chemically, electrically, and biologically. Raman spectroscopy demonstrated that the scaffold outer surface contained both PCL and G, with the G component relatively uniformly distributed. Water contact angle measurement demonstrated that as the amount of G in the scaffold increases (0.75–6% w/w), hydrophobicity decreases; mean contact angle for pure PCL was recorded as 107.22 ± 9.39°, and that with 6% G (PCL+6G) as 77.56 ± 6.75°. Electrochemical Impedance Spectroscopy demonstrated a marked increase in electroactivity potential with increasing G concentration. Cell viability results indicated that even the smallest addition of G (0.75%) resulted in a significant improvement in electroactivity potential and bioactivity compared with that for pure PCL, with 1.5 and 3% exhibiting the highest statistically significant increases in cell proliferation.

Topics

• Deposition
• impedance spectroscopy
• surface
• Raman spectroscopy
• electrical conductivity
• biocompatibility
• bioactivity