• Słota, Dagmara
• Sadlik, Julia
• Tomala, Agnieszka Maria
• Kosińska, Edyta
• Sobczak-Kupiec, Agnieszka
• Niziołek, Karina
• Piątek, Paweł
• Jampilek, Josef
• Włodarczyk, Marcin
• Skibiński, Jakub

Abstract

In an increasingly aging society, there is a growing demand for the development oftechnology related to tissue regeneration. It involves the development of the appropriate biomaterialswhose properties will allow the desired biological response to be obtained. Bioactivity is stronglyaffected by the proper selection of active ingredients. The aim of this study was to produce bioactivehydrogel materials based on hyaluronic acid and collagen modified by the addition of placenta. Thesematerials were intended for use as dressings, and their physicochemical properties were investigatedunder simulated biological environmental conditions. The materials were incubated in vitro indifferent fluids simulating the environment of the human body (e.g., simulated body fluid) and thenstored at a temperature close to body temperature. Using an FT-IR spectrophotometer, the functionalgroups present in the composites were identified. The materials with the added placenta showedan increase in the swelling factor of more than 300%. The results obtained confirmed the potentialof using this material as an absorbent dressing. This was indicated by pH and conductometricmeasurements, sorption, degradation, and surface analysis under an optical microscope. The resultsof the in vitro biological evaluation confirmed the cytosafety of the tested biomaterials. The testedcomposites activate monocytes, which may indicate their beneficial properties in the first phases ofwound healing. The material proved to be nontoxic and has potential for medical use.

Topics

• impedance spectroscopy
• surface
• phase
• composite
• aging
• biomaterials
• aging
• bioactivity