• Vera, Merry Angelyn Tan De
• Haidyrah, Ahmed S.
• Aouak, Taieb
• Alrahlah, Ali
• Khan, Rawaiz
• Al-Owais, Ahmad Abdulaziz

Abstract

<jats:p>A series of poly(ethylene-co-vinyl alcohol)/titanium dioxide (PEVAL/TiO2) nanocomposites containing 1, 2, 3, 4 and 5 wt% TiO2 were prepared by the solvent casting method. These prepared hybrid materials were characterized by Fourier-transform infrared (FT-IR), X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM). The pores and their interconnections inside these nanocomposites were created using naphthalene microparticles used as a porogen after having been extracted by sublimation under a high vacuum at temperatures slightly below the glass transition temperature. A cellular activity test of these hybrid materials was performed on human gingival fibroblast cells (HGFs) in accordance with ISO 10993-5 and ISO 10993-12 standards. The bioviability (cell viability) of HGFs was evaluated after 1, 4 and 7 days using Alamar Blue®. The results were increased cell activity throughout the different culture times and a significant increase in cell activity in all samples from Day 1 to Day 7, and all systems tested showed significantly higher cell viability than the control group on Day 7 (p &lt; 0.002). The adhesion of HGFs to the scaffolds studied by SEM showed that HGFs were successfully cultured on all types of scaffolds.</jats:p>

Topics

• nanocomposite
• pore
• scanning electron microscopy
• x-ray diffraction
• glass
• glass
• thermogravimetry
• glass transition temperature
• solvent casting
• differential scanning calorimetry
• casting
• titanium
• alcohol