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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Lieberzeit, Peter
University of Vienna
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (18/18 displayed)
- 2024Novel Approach for the Immobilization of Cellobiose Dehydrogenase in PEDOT:PSS Conductive Layer on Planar Gold Electrodescitations
- 2023Bioanalytische Sensoren auf der Basis von Wärmeleitungeffektencitations
- 2023L-Ascorbic Acid Treatment of Electrochemical Graphene Nanosheets: Reduction Optimization and Application for De-Icing, Water Uptake Prevention, and Corrosion Resistancecitations
- 2022Development of conductive molecularly imprinted polymers (cMIPs) for limonene to improve and interconnect QCM and chemiresistor sensingcitations
- 2021Biomimetic Sensors to Detect Bioanalytes in Real-Life Samples Using Molecularly Imprinted Polymerscitations
- 2021Imprinted polymer and Cu2O-graphene oxide nanocomposite for the detection of disease biomarkerscitations
- 2021Novel dual-sensor for creatinine and 8-hydroxy-2 '-deoxyguanosine using carbon-paste electrode modified with molecularly imprinted polymers and multiple-pulse amperometrycitations
- 2020Molecularly imprinted polymeric coatings for sensitive and selective gravimetric detection of artemethercitations
- 2020Design of heterostructured hybrids comprising ultrathin 2D bismuth tungstate nanosheets reinforced by chloramphenicol imprinted polymers used as biomimetic interfaces for mass-sensitive detectioncitations
- 2020Novel amino-containing molecularly-imprinted polymer coating on magnetite-gold core for sensitive and selective carbofuran detection in foodcitations
- 2019Highly sensitive and selective electrochemical paper-based device using a graphite screen-printed electrode modified with molecularly imprinted polymers coated Fe3O4@Au@SiO2 for serotonin determinationcitations
- 2018Investigating nanohybrid material based on 3D CNTs@Cu nanoparticle composite and imprinted polymer for highly selective detection of chloramphenicolcitations
- 2017A novel method for dengue virus detection and antibody screening using a graphene-polymer based electrochemical biosensorcitations
- 2016A Self-Organisation Synthesis Approach for Bacteria Molecularly Imprinted Polymerscitations
- 2014Molecularly imprinted polymer-Ag2S nanoparticle composites for sensing volatile organicscitations
- 2009Generating Bio-Analogous Recognition of Artificial Materials - Sensors and Electronic Noses for Odours
- 2007Printing materials in micro- and nano-scale: Systems for process control
- 2003Chemical sensors - From molecules, complex mixtures to cells - Supramolecular imprinting strategiescitations
Places of action
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article
Novel Approach for the Immobilization of Cellobiose Dehydrogenase in PEDOT:PSS Conductive Layer on Planar Gold Electrodes
Abstract
<jats:p>Third-generation biosensors use enzymes capable of direct electron transfer (DET) to the sensor surface. They are of interest for continuous glucose monitoring in blood or interstitial fluid, but they are rarely investigated. One reason is the hindered DET of the enzymes to the metallic electrodes. In this publication, a novel method for the immobilization of cellobiose dehydrogenase (CDH) DET enzymes employing conductive poly(3,4-ethylenedioxythiophene)-poly (styrene sulfonate) (PEDOT:PSS) inks and a protective polyethylene glycol dimethacrylate (PEG-DMA) hydrogel layer on gold electrodes is reported. This layer stack showed a glucose-specific current response for voltages between −0.2 and 0.4 V in physiological PBS buffer, and enabled interference-less sensing in a solution of acetaminophen, ascorbic acid, dopamine, and uric acid at 0 V. A Michaelis–Menten fit led to a maximum current density (Imax) of 257 ± 7.9 nA/mm2 and a Michaelis–Menten constant (Km) of 28.4 ± 2.2 mM, with a dynamic range of 0.1–20 mM glucose and a limit of detection of 0.1 mM. After 16 h of continuous measurement of 20 mM glucose, the signal decreased to 60% of its initial value. Storage stability was successfully verified until up to 10 days. In summary, this paper shows a simplified approach for the fabrication of third-generation biosensors using CDH-PEDOT:PSS and PEG-DMA hydrogel inks.</jats:p>