Materials Map

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The Materials Map is an open tool for improving networking and interdisciplinary exchange within materials research. It enables cross-database search for cooperation and network partners and discovering of the research landscape.

The dashboard provides detailed information about the selected scientist, e.g. publications. The dashboard can be filtered and shows the relationship to co-authors in different diagrams. In addition, a link is provided to find contact information.

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The Materials Map is still under development. In its current state, it is only based on one single data source and, thus, incomplete and contains duplicates. We are working on incorporating new open data sources like ORCID to improve the quality and the timeliness of our data. We will update Materials Map as soon as possible and kindly ask for your patience.

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in Cooperation with on an Cooperation-Score of 37%

Topics

Publications (1/1 displayed)

  • 2022Reliable and Fast Genotyping Protocol for Galactosylceramidase (Galc) in the Twitcher (Twi) Mouse3citations

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Parlanti, Gabriele
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Tonazzini, Ilaria
1 / 1 shared
Carpi, Sara
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Scaccini, Luca
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Del Grosso, Ambra
1 / 1 shared
Sarlo, Miriam De
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Cecchini, Marco
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2022

Co-Authors (by relevance)

  • Parlanti, Gabriele
  • Tonazzini, Ilaria
  • Carpi, Sara
  • Scaccini, Luca
  • Del Grosso, Ambra
  • Sarlo, Miriam De
  • Cecchini, Marco
OrganizationsLocationPeople

article

Reliable and Fast Genotyping Protocol for Galactosylceramidase (Galc) in the Twitcher (Twi) Mouse

  • Parlanti, Gabriele
  • Tonazzini, Ilaria
  • Carpi, Sara
  • Scaccini, Luca
  • Del Grosso, Ambra
  • Sarlo, Miriam De
  • Colagiorgio, Laura
  • Cecchini, Marco
Abstract

<jats:p>Twitcher (Twi) is a neurological Krabbe disease (KD, or globoid cell leukodystrophy) spontaneous mutant line in mice. The genome of the Twi mouse presents a single nucleotide polymorphism (SNP), leading to an enzymatically inactive galactosylceramidase (Galc) protein that causes KD. In this context, mouse Twi genotyping is an essential step in KD research. To date, the genotyping method used is labor-intensive and often has ambiguous results. Here, we evaluated a novel protocol for the genotype determination of Galc mutation status in Twi mice based on the allele-discrimination real-time polymerase chain reaction (PCR). Here, DNA is extracted from Twi mice (n = 20, pilot study; n = 120, verification study) and control group (n = 10, pilot study; n = 30 verification study) and assessed by allele-discrimination real-time PCR to detect SNP c.355G&gt;A. Using the allele-discrimination PCR, all of the samples are identified correctly with the genotype GG (wild-type, WT), GA (heterozygote, HET), or AA (homozygote, HOM) using the first analysis and no animals are not genotyped. We demonstrated that this novel method can be used to distinguish KD timely, accurately, and without ambiguity in HOM, WT, and HET animals. This protocol represents a great opportunity to increase accuracy and speed in KD research.</jats:p>

Topics
  • impedance spectroscopy