• Nadri, S.
• Zare, S.
• Mohammadpour, M.
• Ghazanfari, Samaneh
• Izadi, Z.
• Samadian, H.

Abstract

Simple Summary Currently, bone defects, diseases, and injuries are common and global problems. These defects can be treated with several surgical methods and bone grafting, but these methods have limitations, including immune disorders, risk of infection, long-term recovery, movement problems, and high costs. A promising treatment option for bone replacement is the design and construction of scaffolds that mimic the properties of bone tissue and provide a suitable environment for cell and tissue growth. Achieving successful results in this method is dependent on the composition and structure of materials used as scaffolds. Bone is a composite consisting of a mineral fraction, mainly a combination of calcium phosphate, and an organic matrix. Here, we designed and produced a porous, non-toxic, and degradable scaffold made of alginate natural polymer and bioactive glass that contains strontium as well as the common elements of bioglasses-silica, calcium, sodium, and phosphorus. The scaffold is degraded at an optimized rate with the simultaneous proliferation and growth of cells, thus providing a suitable environment for the growth and development of new tissue and blood vessels. The outcomes of this study presented this scaffold as a functional structure to be used in treating bone defects and reconstructing damaged bone. The main aim of the current study is to fabricate an osteocompatible, bioactive, porous, and degradable bone tissue engineering scaffold. For this purpose, bioactive glasses (BGs) were chosen due to their similarity to bone's natural mineral composition, and the effect of replacing Ca ions with Sr on their properties were considered. First, strontium-containing BGs (Sr-BGs) were synthesized using the electrospinning technique and assembled by the sol-gel method, then they were incorporated into the alginate (Alg) matrix. Photographs of the scanning electron microscope (SEM) showed that the BG nanofibers have a diameter of 220 +/- 36 nm, which was smaller than the precursor nanofibers (275 +/- 66 nm). The scaffolds possess a porous internal microstructure (230-330 nm pore size) with interconnected pores. We demonstrated that the scaffolds could be degraded in the acetate sodium buffer and phosphate-buffered saline. The osteoactivity of the scaffolds was confirmed via visual inspection of the SEM illustrations after seven days of immersing them in the SBF solution. In vitro assessments disclosed that the produced Alg-based composites including Sr-BGs (Alg/Sr-BGs) are blood-compatible and biocompatible. Accumulating evidence shows that Alg/Sr-BG (5%, 10%, and 15%) hydrogels could be a promising scaffold for bone regeneration.

Topics

• porous
• nanocomposite
• impedance spectroscopy
• microstructure
• pore
• mineral
• polymer
• scanning electron microscopy
• glass
• glass
• Sodium
• Strontium
• Calcium
• electrospinning
• Phosphorus