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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Rodríguez, Sebastián
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article
An Analytical–Experimental Approach to Quantifying the Effects of Static Magnetic Fields for Cell Culture Applications
Abstract
<jats:p>This work aimed to study the effects of static magnetic fields (SMFs) on cell cultures. A glass flask was filled with a liquid medium, which was surrounded by permanent magnets. Air was introduced through a tube to inject bubbles. Two magnet configurations, north and south, were used as perturbation. Scenedesmus obliquus and Nannochloropsis gaditana, growing in Medium 1 and 2, were subjected to the bubbly flow and SMFs. Differences between media were mainly due to conductivity (0.09 S/m for Medium 1 and 4.3 S/m for Medium 2). Joule dissipation (P) increased with the magnetic flux density (B 0), being 4 orders of magnitude higher in Medium 2 than in 1. Conversely, the time constant (τ P) depended onB 0, being nearly constant for Medium 1 and decreasing at 449 s/T for Medium 2. Dissipation occurred with the sameτ P(235 s) in Medium 1 and 2 atB 0= 0.5T. In Species 1, the SMF effect was inhibitory. For Species 2, a higher enzymatic activity was observed. For superoxide dismutase, the relative difference was 78% with the north and 115% with the south configuration compared to the control values. For the catalase, differences of 29% with the north and 23% with the south configuration compared to control condition were obtained.</jats:p>