• Ounnunkad, Kontad
• Jakmunee, Jaroon

Abstract

<jats:p>A bi-functional material based on silver nanoparticles (AgNPs)-reduced graphene oxide (rGO) composite for both electrode modification and signal generation is successfully synthesized for use in the construction of a label-free electrochemical immunosensor. An AgNPs/rGO nanocomposite is prepared by a one-pot wet chemical process. The AgNPs/rGO composite dispersion is simply cast on a screen-printed carbon electrode (SPCE) to fabricate the electrochemical immunosensor. It possesses a sufficient conductivity/electroreactivity and improves the electrode reactivity of SPCE. Moreover, the material can generate an analytical response due to the formation of immunocomplexes for detection of human immunoglobulin G (IgG), a model biomarker. Based on electrochemical stripping of AgNPs, the material reveals signal amplification without external redox molecules/probes. Under optimized conditions, the square wave voltammetric peak current is responded to the logarithm of IgG concentration in two wide linear ranges from 1 to 50 pg.ml<jats:sup>−1</jats:sup> and 0.05 to 50 ng.ml<jats:sup>−1</jats:sup>, and the limit of detection (LOD) is estimated to be 0.86 pg.ml<jats:sup>−1</jats:sup>. The proposed immunosensor displays satisfactory sensitivity and selectivity. Importantly, detection of IgG in human serum using the immunosensor shows satisfactory accuracy, suggesting that the immunosensor possesses a huge potential for further development in clinical diagnosis.</jats:p>

Topics

• nanoparticle
• nanocomposite
• impedance spectroscopy
• dispersion
• Carbon
• silver