| People | Locations | Statistics |
|---|---|---|
| Naji, M. |
| |
| Motta, Antonella |
| |
| Aletan, Dirar |
| |
| Mohamed, Tarek |
| |
| Ertürk, Emre |
| |
| Taccardi, Nicola |
| |
| Kononenko, Denys |
| |
| Petrov, R. H. | Madrid |
|
| Alshaaer, Mazen | Brussels |
|
| Bih, L. |
| |
| Casati, R. |
| |
| Muller, Hermance |
| |
| Kočí, Jan | Prague |
|
| Šuljagić, Marija |
| |
| Kalteremidou, Kalliopi-Artemi | Brussels |
|
| Azam, Siraj |
| |
| Ospanova, Alyiya |
| |
| Blanpain, Bart |
| |
| Ali, M. A. |
| |
| Popa, V. |
| |
| Rančić, M. |
| |
| Ollier, Nadège |
| |
| Azevedo, Nuno Monteiro |
| |
| Landes, Michael |
| |
| Rignanese, Gian-Marco |
|
Perriman, Adam Willis
University of Bristol
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (17/17 displayed)
- 2024Flax fibre reinforced alginate poloxamer hydrogelcitations
- 2023A rapid high throughput bioprinted colorectal cancer spheroid platform for in vitro drug- and radiation-responsecitations
- 2023Bienzymatic Generation of Interpenetrating Polymer Networked Engineered Living Materials with Shape Changing Propertiescitations
- 2023Design space and manufacturing of programmable 4D printed continuous flax fibre polylactic acid composite hygromorphscitations
- 2022The influence of the humidity on the mechanical properties of 3D printed continuous flax fibre reinforced poly(lactic acid) compositescitations
- 2022The Design of 4D-Printed Hygromorphscitations
- 2021Multiphase lattice metamaterials with enhanced mechanical performancecitations
- 2021Three-Dimensional Printable Enzymatically Active Plasticscitations
- 2020Chondroinduction of Mesenchymal Stem Cells on Cellulose-Silk Composite nanofibrous Substratescitations
- 2020Cactus-based solids and bio-composites for energy dissipation in defence and biomedical applications.
- 2020Abnormal stiffness behaviour in artificial cactus-inspired reinforcement materialscitations
- 2019A Composite Hydrogel Scaffold Permits Self‐Organization and Matrix Deposition by Cocultured Human Glomerular Cellscitations
- 2019Sequential Electrostatic Assembly of a Polymer Surfactant Corona Increases Activity of the Phosphotriesterase arPTEcitations
- 2017Mechanics and band gaps in hierarchical auxetic rectangular perforated composite metamaterialscitations
- 2014Molecular dynamics simulations reveal a dielectric-responsive coronal structure in protein-polymer surfactant hybrid nanoconstructscitations
- 2012Polymer/nucleotide droplets as bio-inspired functional micro-compartmentscitations
- 2009Membrane stabilization and transformation in organoclay-vesicle hybrid constructscitations
Places of action
| Organizations | Location | People |
|---|
article
Chondroinduction of Mesenchymal Stem Cells on Cellulose-Silk Composite nanofibrous Substrates
Abstract
Smart biomaterials with an inherent capacity to elicit specific behaviours in lieu of biological prompts are advantageous for regenerative medicine applications. In this work, we employ an electrospinning technique to model the in vivo nanofibrous extracellular matrix (ECM) of cartilage using a chondroinductive cellulose and silk polymer blend (75:25 ratio). This natural polymer composite is directly electrospun for the first time, into nanofibers without post-spun treatment, using a trifluoroacetic acid and acetic acid cosolvent system. Biocompatibility of the composite with human mesenchymal stem cells (hMSCs) is demonstrated and its inherent capacity to direct chondrogenic stem cell differentiation, in the absence of stimulating growth factors, is confirmed. This chondrogenic stimulation could be countered biochemically using fibroblast growth factor-2, a growth factor used to enhance the proliferation of hMSCs. Furthermore, the potential mechanisms driving this chondroinduction at the cell-biomaterial interface were investigated. Composite substrates were fabricated as two-dimensional film surfaces and cultured with hMSCs in the presence of chemicals that interfere with their biochemical and mechanical signalling pathways. Preventing substrate surface elasticity transmission resulted in significant downregulation of chondrogenic gene expression. Interference with the classical chondrogenic Smad2/3 phosphorylation pathway did not impact chondrogenesis. The results highlight the importance of substrate mechanical elasticity on hMSCs chondroinduction and its independence to known chondrogenic biochemical pathways. The newly fabricated scaffolds provide the foundation for designing a robust, self-inductive, and cost-effective biomimetic, natural biomaterial for cartilage tissue engineering.