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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Su, Bo
University of Bristol
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (29/29 displayed)
- 2024Nanotextured titanium inhibits bacterial activity and supports cell growth on 2D and 3D substrate: A co-culture study.citations
- 2023Bio-inspired nacre-like zirconia/PMMA composites for chairside CAD/CAM dental restorationscitations
- 2022Supramolecular Protein Stabilization with Zwitterionic Polypeptide-Cucurbit[7]uril Conjugates.citations
- 2021Friction at nanopillared polymer surfaces beyond Amontons' laws:Stick-slip amplitude coefficient (SSAC) and multiparametric nanotribological propertiescitations
- 2021Friction at nanopillared polymer surfaces beyond Amontons' lawscitations
- 2020Chondroinduction of Mesenchymal Stem Cells on Cellulose-Silk Composite nanofibrous Substratescitations
- 2020Chondroinduction of Mesenchymal Stem Cells on Cellulose-Silk Composite nanofibrous Substrates:The Role of Substrate Elasticitycitations
- 2020Characterisation of mechanical and surface properties of novel biomimetic interpenetrating alumina-polycarbonate composite materialscitations
- 2018Bi-directional freeze casting of porous alumina ceramics:a study of the effects of different processing parameters on microstructurecitations
- 2018Fabrication and characterisation of a novel biomimetic anisotropic ceramic/polymer-infiltrated composite materialcitations
- 2018Bi-directional freeze casting of porous alumina ceramicscitations
- 2018Bi-directional freeze casting of porous alumina ceramics: A study of the effects of different processing parameters on microstructurecitations
- 2018Harnessing nanotopography to enhance osseointegration of clinical orthopedic titanium implants-an in vitro and in vivo analysiscitations
- 2017Towards the cell-instructive bactericidal substrate: exploring the combination of nanotopographical features and integrin selective synthetic ligandscitations
- 2017Macroscale and Nanoscale Morphology Evolution during in Situ Spray Coating of Titania Films for Perovskite Solar Cellscitations
- 2017Pore size control of block copolymer-templated sol–gel-synthesized titania films deposited via spray coatingcitations
- 2016Bactericidal nanospike surfaces via thermal oxidation of Ti alloy substratescitations
- 2015Effect of Blend Composition and Additives on the Morphology of PCPDTBT:PC 71 BM Thin Films for Organic Photovoltaicscitations
- 2015Development of the Morphology during Functional Stack Build-up of P3HT:PCBM Bulk Heterojunction Solar Cells with Inverted Geometrycitations
- 2015Arrangement of Maghemite Nanoparticles via Wet Chemical Self-Assembly in PS- b -PNIPAM Diblock Copolymer Filmscitations
- 2014Tailoring titania nanostructures for solar cell applications
- 2014Self-Assembly of Diblock Copolymer–Maghemite Nanoparticle Hybrid Thin Filmscitations
- 2013Critical issues in near net shape forming via green machining of ceramics: A case study of alumina dental crowncitations
- 2013Fabrication and characterization of biomimetic ceramic/polymer composite materials for dental restorationcitations
- 2012Tailored graded pore structure in zirconia toughened alumina ceramics using double-side cooling freeze castingcitations
- 2007Biomimetic apatite/polycaprolactone (PCL) nanofibres for bone tissue engineering scaffolds
- 2006Spatial distribution of polarization and space charge in barium strontium titanate ceramicscitations
- 2006Manufacture and characterization of high activity piezoelectric fibrescitations
- 2004High frequency thick film BST ferroeclectric phase shiftercitations
Places of action
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article
Chondroinduction of Mesenchymal Stem Cells on Cellulose-Silk Composite nanofibrous Substrates
Abstract
Smart biomaterials with an inherent capacity to elicit specific behaviours in lieu of biological prompts are advantageous for regenerative medicine applications. In this work, we employ an electrospinning technique to model the in vivo nanofibrous extracellular matrix (ECM) of cartilage using a chondroinductive cellulose and silk polymer blend (75:25 ratio). This natural polymer composite is directly electrospun for the first time, into nanofibers without post-spun treatment, using a trifluoroacetic acid and acetic acid cosolvent system. Biocompatibility of the composite with human mesenchymal stem cells (hMSCs) is demonstrated and its inherent capacity to direct chondrogenic stem cell differentiation, in the absence of stimulating growth factors, is confirmed. This chondrogenic stimulation could be countered biochemically using fibroblast growth factor-2, a growth factor used to enhance the proliferation of hMSCs. Furthermore, the potential mechanisms driving this chondroinduction at the cell-biomaterial interface were investigated. Composite substrates were fabricated as two-dimensional film surfaces and cultured with hMSCs in the presence of chemicals that interfere with their biochemical and mechanical signalling pathways. Preventing substrate surface elasticity transmission resulted in significant downregulation of chondrogenic gene expression. Interference with the classical chondrogenic Smad2/3 phosphorylation pathway did not impact chondrogenesis. The results highlight the importance of substrate mechanical elasticity on hMSCs chondroinduction and its independence to known chondrogenic biochemical pathways. The newly fabricated scaffolds provide the foundation for designing a robust, self-inductive, and cost-effective biomimetic, natural biomaterial for cartilage tissue engineering.