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| Petrov, R. H. | Madrid |
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| Azevedo, Nuno Monteiro |
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Cooper, Emily
Imperial College London
in Cooperation with on an Cooperation-Score of 37%
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document
Pooling of Nasopharyngeal (NP) Swab Samples to Overcome Global Shortage of rRT-PCR COVID-19 Test Kits
Abstract
<p>The global outbreak and rapid spread of SARS-CoV-2 has created an urgent need for large scale testing of populations.There is a demand for high throughput testing protocols that can be used for efficient and rapid testing of clinical specimens.We evaluated a pooled-PCR protocol for testing nasopharyngeal swabs using known positive/negative and untested clinical samples that were assigned to pools of 5 or 10.Nasopharyngeal swabs were accurately identified as positive or negative for SARS-CoV-2 in pools of 5 (100% sensitivity; 100% specificity).Even though specificity remained unaffected (100%), the detection sensitivity was reduced (66.67%) when 10 samples were pooled together.Pooling of up to 5 samples can be employed in laboratories for the diagnosis of COVID-19 for efficient utilization of resources, rapid screening of a greater number of people, and faster reporting of test results.</p>