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Colovic, Bozana
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article
Nucleation of biomimetic hydroxyapatite
Abstract
<jats:p>Introduction. The aim of the study was to assess the formation of biomimeticcalcium hydroxyapatite (HAP) on the surface of different substrates. Materialand Methods. Silica coated stainless steel tapes and thin polymer films(alginate, cellulose, poly lactide-co-glycolide - PLGA) deposited onhydroxyapatite scaffold were used as substrate. Supersaturated simulated bodyfluid (SBF) and SBF combined with Fetal Calf Serum (FCS) or Eagle?s MinimumEssential Medium (EMEM) were used as bioactive liquid medium where biomimeticnucleation of HAP occurred. Infrared spectroscopy with Fourier transformationwas used to analyze the formed phases, while scanning electron microscopyindicated the morphology of nucleated phase. Results. The results ofmeasuring the mass with volume adjustments done by the BET method showed thatthe thickness of the film of nucleated calcium hydroxyapatite depended onthe time that samples spent soaked in SBF-in as well as the type of selectedbiomimetic medium. Conclusion. Biomimetic calcium hydroxyapatite is possibleto produce by self nucleation on different substrates in the presence ofsimulating body fluid.</jats:p>