• Grubisa, Ivana
• Otasevic, Petar
• Dimkovic, Nada
• Toljic, Bosko
• Nedeljkovic, Ivana
• Vucinic, Nada

Abstract

<jats:p>Introduction. Paraoxonase 1 (PON1) is a multifunctional enzyme associatedwith high-density lipoprotein particles (HDL). It is a cellular antioxidantthat hydrolyses oxidized macromolecules, especially low-density lipoproteins(ox-LDL). Because increased oxidative stress is believed to play a crucialrole in the initiation and propagation of atherosclerosis, coding (Q192R andL55M) and promoter (C(-107)T) region polymorphisms of pon1 gene, that areresponsible for catalytic efficiency, activity and the level of the enzyme,have been of great interest as a potential markers of susceptibility foratherogenesis. Objective. The aim of the study was to assess possibleassociation between these pon1 gene variants and clinical manifestations ofthe atherosclerosis and oxidative stress. Methods. A total of 60angiographically documented patients with manifested atherosclerotic diseaseand 100 control individuals were analyzed. Genomic DNA was isolated from theperipheral blood cells and genotyping was performed using polymerase chainreaction followed by the restriction fragment length polymorphism (PCR-RFLP)analysis. Results No significant difference in allele and genotypefrequencies of all three examined polymorphisms was found between theatherosclerotic patients and healthy controls. The obtained results could notsupport an association of pon1 gene variants with the oxidative stress andatherogenesis. Conclusion. These polymorphisms cannot be considered riskfactors of atherosclerosis in Serbian population. A larger study is requiredin order to establish possible contribution of pon1 variants toatherosclerosis-related cardiovascular diseases.</jats:p>

Topics

• density
• impedance spectroscopy
• susceptibility