• Dedic, Velimir
• Despotovic, Nebojsa
• Grubisa, Ivana
• Otasevic, Petar
• Milasin, Jelena
• Vucinic, Nada

Abstract

<jats:p>One of the characteristics of type 2 diabetes mellitus (T2DM) is the state ofpersistent oxidative stress (OS) that has been implicated in the pathogenesisof diseases such is atherosclerosis mainly through chronic hyperglycemia thatstimulates production of reactive oxygen species (ROS) and increases OS.Glutathione S-transferase P1 (GSTP1) is a member of the cytosolic GSTsuperfamily. It plays an important role in neutralizing OS as an enzyme.Also, it participates in regulation of stress signaling and protects cellsagainst apoptosis via its noncatalytic ligand-binding activity. GSTP1Ile105Val functional polymorphism influences protein catalytic activity andstability and the aim of this study was to determine whether this genevariation influences susceptibility to atherogenesis in T2DM patients. Atotal of 240 individuals (140 patients with T2DM, accompanied with clinicalmanifestations of atherosclerosis, and 100 healthy controls) were included inthis study. Genomic DNA was isolated from peripheral blood cells andgenotyping was performed using polymerase chain reaction followed byrestriction fragment length polymorphism (PCR-RFLP) analysis. We obtained nostatistically significant differences in the distribution of alleles andgenotypes between cases and controls (P&gt;0.05) but association between Ile/Val(OR=0.6, 95%CI=0.35-1.05, P=0.08) and Val/Val (OR=0.45, 95%CI=0.18-1.11,P=0.08) genotypes and disease approached significance (P=0.08). Our resultsindicated that a larger study group is needed to establish the truerelationship between potentialiy protective allele Val and the disease, andto determine the influence of other GSTP1 polymorphisms on atherogenesis inT2DM patients.</jats:p>

Topics

• impedance spectroscopy
• Oxygen
• reactive
• susceptibility
• chemical ionisation