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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Neves, Luísa A.
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Publications (6/6 displayed)
- 2024A greener route to prepare PEBAX®1074 membranes for gas separation processescitations
- 2022Deep eutectic systems for carbonic anhydrase extraction from microalgae biomass to improve carbon dioxide solubilizationcitations
- 2021Modelling CO2 absorption in aqueous solutions of cholinium lysinate ionic liquidcitations
- 2019Demonstration of the adhesive properties of the medium-chain-length polyhydroxyalkanoate produced by Pseudomonas chlororaphis subsp. aurantiaca from glycerolcitations
- 2019Production of medium-chain length polyhydroxyalkanoates by Pseudomonas citronellolis grown in apple pulp wastecitations
- 2017Membranes with a low loading of Metal–Organic Framework-Supported Ionic Liquids for CO2/N2 separation in CO2 capturecitations
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article
Production of medium-chain length polyhydroxyalkanoates by Pseudomonas citronellolis grown in apple pulp waste
Abstract
<p>Background and objective: Apple pulp waste generated by the fruit processing industry is a sugar-rich material with great potential to be used as a feedstock for production of valueadded microbial products. The aim of this work was to use this feedstock for the cultivation of Pseudomonas citronellolis and production of medium-chain-length polyhydroxyalkanoates, a natural elastomer. Material and methods: The solid fraction of the apple pulp waste was discarded and the soluble fraction, rich in fructose (17.7 g l<sup>-1</sup>), glucose (7.5 g l<sup>-1</sup>) and sucrose (1.2 g l-1), was used for the batch bioreactor cultivation of Pseudomonas citronellolis NRRL B-2504. Results and conclusion: Pseudomonas citronellolis reached a polymer content in the biomass of 30% wt and a volumetric productivity of 0.025 g l<sup>-1</sup>h<sup>-1</sup>. The polymer was mainly composed of 3-hydroxydecanoate (68% mol) and 3-hydroxyoctanoate (22% mol), with minor contents of 3-hydroxydodecanoate (5% mol), 3-hydroxytetradecanoate (4% mol) and 3-hydroxyhexanoate (1% mol), and had a molecular weight of 3.7×10<sup>5</sup>Da. It presented glass transition and melting temperatures of -12 and 53°C, respectively, and a thermal degradation temperature of 296°C. The polymer's films were dense, ductile and permeable to oxygen and carbon dioxide. These results demonstrated that apple pulp waste is a suitable feedstock for the production of a biopolymer with properties that render it a promising alternative to some synthetic petrochemical-derived polyesters. Conflict of interest: The authors declare no conflict of interest.</p>