• Saliev, T.
• Melzer, Andreas
• Karakitsios, I.
• Ahmad, S.
• Donald-Simpson, Helen
• Eisma, R.

Abstract

Introduction<br/>Thielembalmingtechniqueprovidesanidealmedium forconservationofcadaversandorgansforfurther experimentationandtestingdevicesfornon-invasivesurgery,includingMR-guidedFocusedUltrasound (MRgFUS). The rational of this studywas to scrutinize the response of Thiel-embalmed tissue of explanted human liver and kidney on different acoustic energies.<br/><br/>Methods<br/>ThesonicationwasperformedbyMRgFUS(ExAblate,InSightec).Afterpre-planningMR-imagingand defining regions of treatment, the tissue was ablated by ultrasound with frequency of 1.15 MHz. Three spots on the liver were sonicated with acoustic energies of 253 J, 506 J and 709 J respectively (time 20 sec). The kidney was sonicated on three different locations. First spot was treated with energy of 777 J (40 sec), second spot with 863 J (40 sec). The last spot on kidney was treated 3 times consistently with acoustic energy of 777 J (120 sec). <br/><br/>Results<br/>The obtained data demonstrated that sonication of the liver at ‘low’ regimeinduced the rise of temperature up to59C,whilethe‘medium’and‘high’regimeselicitedsignificantlyhigherriseupto109Cand120C respectively. The prolonged treatment of the same spot of the liver did not lead to increase of temperature due to inductionofcavitation(‘screeningeffect’).Thesameeffectwasobservedforthekidney,wheredespiteof continuous sonication of the same spot, the maximal temperature did not exceed 75 C. The highest temperature rise for kidney was 87 C (at 863 J). <br/><br/>Conclusion<br/>The acquired data indicate that Thiel embalmed organs constitute a suitable model for testing and optimization of MRgFUS.

Topics

• size-exclusion chromatography