| People | Locations | Statistics |
|---|---|---|
| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Bouten, Cvc Carlijn
Eindhoven University of Technology
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (13/13 displayed)
- 2023How Smart are Smart Materials?citations
- 2020Optimization of Anti-kinking Designs for Vascular Grafts Based on Supramolecular Materialscitations
- 2020Imaging the In Vivo Degradation of Tissue Engineering Implants by Use of Supramolecular Radiopaque Biomaterialscitations
- 2019Macrophage-driven biomaterial degradation depends on scaffold microarchitecturecitations
- 2018Intrinsic cell stress is independent of organization in engineered cell sheetscitations
- 2017Biomaterial-driven in situ cardiovascular tissue engineering : a multi-disciplinary perspectivecitations
- 2017Porous scaffolds using dual electrospinning for in situ cardiovascular tissue engineeringcitations
- 2017Mechanically robust electrospun hydrogel scaffolds crosslinked via supramolecular interactionscitations
- 2015Hydrolytic and oxidative degradation of electrospun supramolecular biomaterialscitations
- 2015Hydrolytic and oxidative degradation of electrospun supramolecular biomaterials:In vitro degradation pathwayscitations
- 2014Monocytic cells become less compressible but more deformable upon activationcitations
- 2013Mechanical analysis of ovine and pediatric pulmonary artery for heart valve stent designcitations
- 2003Finite element model of mechanically induced collagen fiber synthesis and degradation in the aortic valvecitations
Places of action
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article
Monocytic cells become less compressible but more deformable upon activation
Abstract
AimsMonocytes play a significant role in the development of atherosclerosis. During the process of inflammation, circulating monocytes become activated in the blood stream. The consequent interactions of the activated monocytes with the blood flow and endothelial cells result in reorganization of cytoskeletal proteins, in particular of the microfilament structure, and concomitant changes in cell shape and mechanical behavior. Here we investigate the full elastic behavior of activated monocytes in relation to their cytoskeletal structure to obtain a better understanding of cell behavior during the progression of inflammatory diseases such as atherosclerosis. Methods and Results The recently developed Capillary Micromechanics technique, based on exposing a cell to a pressure difference in a tapered glass microcapillary, was used to measure the deformation of activated and non-activated monocytic cells. Monitoring the elastic response of individual cells up to large deformations allowed us to obtain both the compressive and the shear modulus of a cell from a single experiment. Activation by inflammatory chemokines affected the cytoskeletal organization and increased the elastic compressive modulus of monocytes with 73–340%, while their resistance to shape deformation decreased, as indicated by a 25–88% drop in the cell’s shear modulus. This decrease in deformability is particularly pronounced at high strains, such as those that occur during diapedesis through the vascular wall. ConclusionOverall, monocytic cells become less compressible but more deformable upon activation. This change in mechanical response under different modes of deformation could be important in understanding the interplay between the mechanics and function of these cells. In addition, our data are of direct relevance for computational modeling and analysis of the distinct monocytic behavior in the circulation and the extravascular space. Lastly, an understanding of the changes of monocyte mechanical properties will be important in the development of diagnostic tools and therapies concentrating on circulating cells.