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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Naebe, Minoo
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (9/9 displayed)
- 2019Low-Cost Carbon Fibre Derived from Sustainable Coal Tar Pitch and Polyacrylonitrile: Fabrication and Characterisationcitations
- 2019Thermodynamic approach to tailor porosity in piezoelectric polymer fibers for application in nanogeneratorscitations
- 2018On the detection of carbon fibre storage contamination and its effect on the fibre–matrix interfacecitations
- 2013Enzyme Immobilisation on Amino-Functionalised Multi-Walled Carbon Nanotubescitations
- 2012Preparation and properties of stepwise graded synthetic graphite/phenolic nanocomposites
- 2012Effect of compositional gradient on thermal behavior of synthetic graphite-phenolic nanocompositescitations
- 2012Composition-optimized synthetic graphite/polymer nanocomposites
- 20113-D seamless knitted preforms for automotive seating applications
- 20113-D Seamless Knitted Preforms for Automotive Seating Applications
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article
Enzyme Immobilisation on Amino-Functionalised Multi-Walled Carbon Nanotubes
Abstract
<p>Background:The aim of this work is to investigate the structure and function of enzymes immobilised on nanomaterials. This work will allow better understanding of enzyme-nanomaterial interactions, as well as designing functional protein-nanomaterial conjugates.Methodology/Principal Findings:Multiwalled carbon nanotubes (MWNTs) were functionalised with amino groups to improve solubility and biocompatibility. The pristine and functionalised forms of MWNTs were characterised with Fourier-transform infrared spectroscopy. Thermogravimetric analysis was done to examine the degree of the functionalisation process. An immobilised biocatalyst was prepared on functionalised nanomaterial by covalent binding. Thermomyces lanuginosus lipase was used as a model enzyme. The structural change of the immobilised and free lipases were characterised with transmission electron Microscopy, X-ray photoelectron spectroscopy, Fourier-transform infrared spectroscopy and Circular dichroism spectroscopy. Biochemical characterisation of immobilised enzyme showed broader pH and thermal optima compared to soluble form. Reusability of the immobilised enzyme for hydrolysis of long chain esters was demonstrated up to ten cycles.Conclusion/Significance:Lipase immobilised on MWNTs has exhibited significantly improved thermal stability. The exploration of advanced nanomaterial for enzyme immobilisation support using sophisticated techniques makes nanobiocatalyst of potential interest for biosensor applications.</p>