• Sakamoto, Noriho
• Kubota, Hiroshi
• Yokota, Shin-Ichi
• Mukae, Hiroshi
• Fujii, Nobuhiro
• Kadota, Jun-Ichi
• Matsuoka, Yasuhiro
• Mizunoe, Syunji
• Kohno, Shigeru
• Nagata, Kazuhiro

Abstract

<jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>The pathological diagnosis of idiopathic interstitial pneumonias (IIP) by surgical lung biopsy is important for clinical decision-making. However, there is a need to use less invasive biomarkers to differentiate nonspecific interstitial pneumonia (NSIP) from other IIP such as usual interstitial pneumonia (UIP). Heat shock protein (HSP) 47, a collagen-specific molecular chaperone, is involved in the processing and/or secretion of procollagen. HSP47 is increased in various fibrotic diseases. We investigated the autoantibodies to HSP47 in IIPs.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>We measured the serum levels of the autoantibodies to HSP47 in 38 patients with various forms of IIP [16 with idiopathic pulmonary fibrosis (IPF), 15 with idiopathic NSIP, 7 with cryptogenic organizing pneumonia (COP)] and 18 healthy volunteers.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>The serum levels of autoantibodies to HSP47 in patients with idiopathic NSIP were significantly higher than in patients with IPF (P &lt; 0.01), COP (P &lt; 0.05), and healthy volunteers (P &lt; 0.05). In addition, those in fibrosing NSIP were significantly higher than those of cellular and fibrosing NSIP (p &lt; 0.05).</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>We found high levels of anti-HSP47 autoantibody titers in sera of patients with idiopathic fibrosing NSIP compared with other IIPs and healthy volunteers.</jats:p></jats:sec>

Topics

• impedance spectroscopy
• laser emission spectroscopy
• interstitial
• size-exclusion chromatography