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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Xu, J.
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (23/23 displayed)
- 2024Injectable Tissue-Specific Hydrogel System for Pulp–Dentin Regenerationcitations
- 2023Cr-doped Al 2 O 3 –YAG binary and Al 2 O 3 –YAG–ZrO 2 ternary eutectic materials crystallized by the micro pulling down technique and their characterization
- 2022Thermochromic Narrow Band Gap Phosphors for Multimodal Optical Thermometry: The Case of Y3+-Stabilized β-Bi2O3:Nd3+citations
- 2021Adhesive metallization on carbon-fiber-reinforced polymer (CFRP) by cold plasma spraying
- 2019Electrocatalytic water oxidation over AlFe 2 B 2citations
- 2018Evidence for a dynamical ground state in the frustrated pyrohafnate Tb 2 Hf 2 O 7citations
- 2016Inducing Elasticity through Oligo-Siloxane Crosslinks for Intrinsically Stretchable Semiconducting Polymers
- 2016A comparative study of twill weave reinforced composites under tension-tension fatigue loading: Experiments and meso-modellingcitations
- 2016Experimental characterization of triaxially braided composites
- 2015A progressive damage model of textile composites on meso-scale using finite element method: Fatigue damage analysiscitations
- 2013Fatigue and post-fatigue stress-strain analysis of a 5-harness satin weave carbon fibre reinforced compositecitations
- 2012In-situ local strain measurement in textile composites with embedded optical fibre sensors
- 2011Local strain variation in the plies of a satin weave composite: experimental vs. numerical
- 2011Local strain in a 5-harness satin weave composite under static tension: Part II - Meso-FE analysiscitations
- 2011Local Strain In A 5 - Harness Satin Weave Composite Under Static Tension: Part Ii - Meso-Fe Analysiscitations
- 2011Local strain in a 5-harness satin weave composite under static tension: Part I - Experimental analysiscitations
- 2010Local damage in a 5-harness satin weave composite under static tension: Part II - Meso-FE modellingcitations
- 2010Evaluation of local strain profiles in a satin weave composite: experimental vs meso-Fe modelling
- 2010Local damage in a 5 - harness satin weave composite under static tension: part i - experimental analysiscitations
- 2010Influence of the Internal Yarn Nesting (Shifting) on the Local Structural Response of a Satin Weave Composite-An Experimental and Numerical Overview
- 2010FE-Modeling of Damage of Twill Carbon/Epoxy Composite on Meso-Scale, Materials Characterization and Experimental Verification
- 2008Elastic and inelastic deformation properties of free standing ceramic EB-PVD coatings
- 2007Influence of the activator in an acrylic bone cement on an array of cement propertiescitations
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article
Injectable Tissue-Specific Hydrogel System for Pulp–Dentin Regeneration
Abstract
<jats:p> The quest for finding a suitable scaffold system that supports cell survival and function and, ultimately, the regeneration of the pulp–dentin complex remains challenging. Herein, we hypothesized that dental pulp stem cells (DPSCs) encapsulated in a collagen-based hydrogel with varying stiffness would regenerate functional dental pulp and dentin when concentrically injected into the tooth slices. Collagen hydrogels with concentrations of 3 mg/mL (Col3) and 10 mg/mL (Col10) were prepared, and their stiffness and microstructure were assessed using a rheometer and scanning electron microscopy, respectively. DPSCs were then encapsulated in the hydrogels, and their viability and differentiation capacity toward endothelial and odontogenic lineages were evaluated using live/dead assay and quantitative real-time polymerase chain reaction. For in vivo experiments, DPSC-encapsulated collagen hydrogels with different stiffness, with or without growth factors, were injected into pulp chambers of dentin tooth slices and implanted subcutaneously in severe combined immunodeficient (SCID) mice. Specifically, vascular endothelial growth factor (VEGF [50 ng/mL]) was loaded into Col3 and bone morphogenetic protein (BMP2 [50 ng/mL]) into Col10. Pulp–dentin regeneration was evaluated by histological and immunofluorescence staining. Data were analyzed using 1-way or 2-way analysis of variance accordingly (α = 0.05). Rheology and microscopy data revealed that Col10 had a stiffness of 8,142 Pa with a more condensed and less porous structure, whereas Col3 had a stiffness of 735 Pa with a loose microstructure. Furthermore, both Col3 and Col10 supported DPSCs’ survival. Quantitative polymerase chain reaction showed Col3 promoted significantly higher von Willebrand factor (VWF) and CD31 expression after 7 and 14 d under endothelial differentiation conditions ( P < 0.05), whereas Col10 enhanced the expression of dentin sialophosphoprotein (DSPP), alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), and collagen 1 (Col1) after 7, 14, and 21 d of odontogenic differentiation ( P < 0.05). Hematoxylin and eosin and immunofluorescence (CD31 and vWF) staining revealed Col10+Col3+DPSCs+GFs enhanced pulp–dentin tissue regeneration. In conclusion, the collagen-based concentric construct modified by growth factors guided the specific lineage differentiation of DPSCs and promoted pulp–dentin tissue regeneration in vivo. </jats:p>